Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,135 results for: URE

Showing: 11971 - 12000 of 18135 results

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pET28a_6HT-TBP6.7(R47A)

Plasmid

#112722

Purpose

Arg47 within TBP6.7 is the most critical residue in terms of forming interactions with WT TAR. Mutating this amino acid to Ala results in ~600-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R47K)

Plasmid

#112723

Purpose

Arg47 of TBP6.7 interacts with Hoogsteen edge of TAR's Gua26. Mutating this amino acid to Lys reduces binding to the WT TAR RNA ~330-fold.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R47K)

Tags

6His-TEV

Expression

Bacterial

Mutation

R47K

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(Q48A)

Plasmid

#112724

Purpose

Gln48 contacts the backbone of TAR RNA as well as mediates intramoecular interaction to stabilize the conformation of beta2-beta3 loop within TBP6.7.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(Q48A)

Tags

6His-TEV

Expression

Bacterial

Mutation

Q48A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(T50A)

Plasmid

#112727

Purpose

Lav-evolved amino acid T50 within TBPs is important in engaging intramolecularly to steer also lab-evolved R49 into conformation compatible binding to RNA.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(T50A)

Tags

6His-TEV

Expression

Bacterial

Mutation

T50A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R52A)

Plasmid

#112729

Purpose

52nd position is Arg in both wild-type U1A and TBPs, but the RNA recognition by this residue in both types of proteins is fundamentally different.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R52A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R52A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(delta C-term)

Plasmid

#112731

Purpose

This mutant of TBP6.7 is lacking residues 91-98 at C-terminus, and was used to test the involvement of those residues in TAR recognition.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(delta C-term)

Tags

6His-TEV

Expression

Bacterial

Mutation

delta C-term

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_FNBP1-1/1

Plasmid

#91552

Purpose

Protein expression and purification of human SH3 domain construct FNBP1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FNBP1-1/1 (FNBP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_ABL2-1/1

Plasmid

#91533

Purpose

Protein expression and purification of human SH3 domain construct ABL2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ABL2-1/1 (ABL2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_CACNB2-2/2

Plasmid

#91507

Purpose

Protein expression and purification of human SH3 domain construct CACNB2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CACNB2-2/2 (CACNB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CD2AP-3/3

Plasmid

#91508

Purpose

Protein expression and purification of human SH3 domain construct CD2AP-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CD2AP-3/3 (CD2AP Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_DLG5-1/1

Plasmid

#91522

Purpose

Protein expression and purification of human SH3 domain construct DLG5-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DLG5-1/1 (DLG5 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_CASKIN1-1/1

Plasmid

#91523

Purpose

Protein expression and purification of human SH3 domain construct CASKIN1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CASKIN1-1/1 (CASKIN1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGAP26-1/1

Plasmid

#91484

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP26-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP26-1/1 (ARHGAP26 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_ITSN2-4/5

Plasmid

#91496

Purpose

Protein expression and purification of human SH3 domain construct ITSN2-4/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN2-4/5 (ITSN2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_ITSN2-1/5

Plasmid

#91497

Purpose

Protein expression and purification of human SH3 domain construct ITSN2-1/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN2-1/5 (ITSN2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_EPS8L1-1/1-IS1/2

Plasmid

#91465

Purpose

Protein expression and purification of human SH3 domain construct EPS8L1-1/1-IS1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

EPS8L1-1/1-IS1/2 (EPS8L1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_ITSN2-3/5

Plasmid

#91470

Purpose

Protein expression and purification of human SH3 domain construct ITSN2-3/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN2-3/5 (ITSN2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_FCHSD2-1/2

Plasmid

#91444

Purpose

Protein expression and purification of human SH3 domain construct FCHSD2-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FCHSD2-1/2 (FCHSD2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_DLG1-1/1

Plasmid

#91448

Purpose

Protein expression and purification of human SH3 domain construct DLG1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DLG1-1/1 (DLG1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_FCHSD1-1/2

Plasmid

#91449

Purpose

Protein expression and purification of human SH3 domain construct FCHSD1-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FCHSD1-1/2 (FCHSD1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_ARHGEF5-1/1

Plasmid

#91452

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF5-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF5-1/1 (ARHGEF5 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_ITSN2-5/5

Plasmid

#91454

Purpose

Protein expression and purification of human SH3 domain construct ITSN2-5/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ITSN2-5/5 (ITSN2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_EFS-1/1

Plasmid

#91456

Purpose

Protein expression and purification of human SH3 domain construct EFS-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

EFS-1/1 (EFS Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_FCHSD2-2/2

Plasmid

#91460

Purpose

Protein expression and purification of human SH3 domain construct FCHSD2-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FCHSD2-2/2 (FCHSD2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_ARHGEF19-1/1

Plasmid

#91432

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF19-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF19-1/1 (ARHGEF19 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_FNBP1L-1/1

Plasmid

#91435

Purpose

Protein expression and purification of human SH3 domain construct FNBP1L-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FNBP1L-1/1 (FNBP1L Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_EPS8L3-1/1

Plasmid

#91395

Purpose

Protein expression and purification of human SH3 domain construct EPS8L3-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

EPS8L3-1/1 (EPS8L3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_MACC1-1/1

Plasmid

#91360

Purpose

Protein expression and purification of human SH3 domain construct MACC1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MACC1-1/1 (MACC1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_ARHGEF38-1/1

Plasmid

#91373

Purpose

Protein expression and purification of human SH3 domain construct ARHGEF38-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGEF38-1/1 (ARHGEF38 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_MACC1-1/1

Plasmid

#91333

Purpose

Protein expression and purification of human SH3 domain construct MACC1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MACC1-1/1 (MACC1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 11971 - 12000 of 18135 results

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