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AiP14132 - pAAV-AiE0779m_3xC2-minBG-jGCaMP8m-WPRE3-BGHpA (Alias: CN4132)

Plasmid

#191735

Purpose

AI plasmid ALIAS: AiP14132. Expression of jGCaMP8m in striatal direct pathway medium spiny neurons (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8m

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13755: pAAV-AiE0743m_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN3755)

Plasmid

#214846

Purpose

Expression of ChR2(H134R)-EYFP in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

ChR2(H134R)-EYFP

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14872: pAAV-AiE0452h_3xC2-minBG-RiboL1-jGCaMP8s-WPRE3-BGHpA (Alias: CN4872)

Plasmid

#214866

Purpose

Expression of RiboL1-jGCaMP8s (soma targeted) in striatal indirect pathway medium spiny neurons (D2 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

RiboL1-jGCaMP8s

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15050 - pAAV-AiE1426m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5050)

Plasmid

#224192

Purpose

AiE1426m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Sst_Chodl neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14361: pAAV-AiE0452h_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4361)

Plasmid

#214857

Purpose

Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

jGCaMP8s

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778)

Plasmid

#199775

Purpose

enhancer AAV for Cre recombinase expression in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

iCre(R297T)

Use

AAV

Mutation

R297T for reduced recombinase efficiency.

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13983: pAAV-AiE0779m_3xC2-minBG-jRGECO1a-WPRE3-BGHpA (Alias: CN3983)

Plasmid

#214850

Purpose

Expression of jRGECO1a in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jRGECO1a

Use

AAV

Mutation

removed NheI, SacI, and MluI sites in jRGECO1a wi…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP13738 - pAAV-AiE0743m_3xC2-minBG-tdTomato-WPRE3-BGHpA (Alias: CN3738)

Plasmid

#224173

Purpose

AiE0743m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV2

Insert

tdTomato

Use

AAV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12447 - pAAV-AiE0617h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2447)

Plasmid

#224164

Purpose

AiE0617h is an enhancer sequence, designed to drive AAV-mediated transgene expression in D2 MSN subtypes (note Olf bulb)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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AiP15148: pAAV-AiE0779m_6xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5148)

Plasmid

#214870

Purpose

Expression of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

CoChR-EGFP

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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