We narrowed to 16,131 results for: form

1. pCSD_2XNLS-SpCas9WT-NLS-3XHA-2XNLS-NmdCas9-NLS

   Plasmid

   #107327

   Purpose

   Expresses SpCas9 fused to dNmCas9 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9-NmCas9 fusion
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   SpCas9 is fused to nuclease dead NmCas9 (D16A, D5…

   Promoter

   CMV IE94

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nrp2.3-AP-His

   Plasmid

   #71974

   Purpose

   Expresses the extracellular region of the Neuropilin 2, isoform 3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Nrp2.3 (Nrp2 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

   Plasmid

   #226350

   Purpose

   Expression of proteasome substrate with tail for molecular ruler

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Expression

   Bacterial

   Mutation

   V15P

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ncam1_1.18.1-Fc-His

   Plasmid

   #72088

   Purpose

   Expresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ncam1_1.18.1 (Ncam1 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSB118 - pL2_pSB90_fLUC-I_HcPro

   Plasmid

   #123193

   Purpose

   binary plant vector for transient TEV HcPro and fLUC (with intron) expression

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Insert

   2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS
   

   Use

   Luciferase

   Expression

   Plant

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MIGR1_FLAG_TAL1-long_GFP

   Plasmid

   #210638

   Purpose

   Overexpression of TAL1-long

   Depositor

   Maayan Salton

   Article

   Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

   Insert

   TAL1-short (TAL1 Human)
   

   Use

   Retroviral

   Available Since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSUPER-retro-puro-shSTIM1

   Plasmid

   #89816

   Purpose

   Knockdown human STIM1 expression

   Depositor

   Shengyu Yang

   Article

   Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

   Insert

   STIM1 shRNA (STIM1 Human)
   

   Use

   Retroviral

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. JE301: pMVP (L5-L4) Tir1-P2A-AID

   Plasmid

   #121722

   Purpose

   pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   osTir1-P2A-AID (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   9x myc (C-term on osTIR1)

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET32a-hBTC

   Plasmid

   #199230

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

   Insert

   Betacellulin (BTC Human)
   

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3

    Plasmid

    #170161

    Purpose

    Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME-P2A-H2B-mRuby3
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. YFP-mDia2

    Plasmid

    #25420

    Depositor

    Arthur Alberts

    Article

    Wallar et al J Biol Chem. 2006 Feb 17. 281(7):4300-7.

    Insert

    diap3 (Diaph3 Mouse)
    

    Tags

    YFP A206K

    Expression

    Mammalian

    Mutation

    Encodes mDia2 amino acids T47 to L1171

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS

    Plasmid

    #107318

    Purpose

    Expresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-SaCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-E6AP Hect

    Plasmid

    #165102

    Purpose

    bacterial expression of a GST fusion of Human E6AP Hect domain

    Depositor

    Arthur Haas

    Article

    Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

    Insert

    E6AP Hect (UBE3A Human)
    

    Tags

    GST from plasmid.

    Expression

    Bacterial

    Mutation

    E6AP Hect domain Amino acids 495-738 (5'QLNP…

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. yPet_cp229-Binder

    Plasmid

    #179137

    Purpose

    YPet circularly permuted at a.a. 229; Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9).

    Depositor

    Klaus Hahn

    Article

    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

    Insert

    SspB
    

    Tags

    yPet_cp229

    Expression

    Mammalian

    Mutation

    Y11K/A15E, residue numbering from pdb: 1ou9

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA

    Plasmid

    #108735

    Purpose

    Expresses N-terminus to aa256 of Cre recombinase fused to FRB CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Cre recombinase fragment N-terminus to aa256
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBW1340_pCAG-PV1-PhiC31-N233-L1-FRB-NLS-BGHpA

    Plasmid

    #108789

    Purpose

    Expresses N-terminus to aa233 of PhiC31 integrase fused to FRB CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment N-terminus to aa233
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FRB,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBW1353_pCAG-PV1-FKBP-L1-PhiC31-234C-NLS-BGHpA

    Plasmid

    #108790

    Purpose

    Expresses aa234 to C-terminus of PhiC31 integrase fused to FKBP CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    PhiC31 integrase fragment aa234 to C-terminus
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    FKBP and NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ntng2.b-AP-His

    Plasmid

    #71995

    Purpose

    Expresses the extracellular region of the Netrin G2, isoform b protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ntng2.b (Ntng2 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-PRAS40 AA

    Plasmid

    #86759

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    human PRAS40 (AKT1S1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AA (S183A, S221A)

    Promoter

    CMV

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Unc5b.a-Fc-His

    Plasmid

    #72178

    Purpose

    Expresses the extracellular region of the Unc5B, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5b.a (Unc5b Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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