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All-in-one split-ADAR2

Plasmid

#170136

Purpose

AAV vector carrying ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

ADAR2-DDC-λN-P2A-MCP-ADAR2-DDN

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pTS111 pHAGE2 CMVtetO2 MCS-3C-22xGS-Alfa-P2A-TagBFP

Plasmid

#199364

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) neo with Ywhag CDS

Plasmid

#61799

Purpose

pcDNA3.1(+) with full length mouse Ywhag CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Ywhag CDS (Ywhag Mouse)

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pFlox c-CTCF FKBPF36V_EYFP_p2aBsd

Plasmid

#163870

Purpose

Acute Depletion of CTCF-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Ctcf (Ctcf Mouse)

Expression

Mammalian

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-hu FceRI beta

Plasmid

#8366

Depositor

Jean Kinet

Article

Kuster et al J Biol Chem 1992 Jun 25;267(18):12782-7.

Insert

human Fc epsilon RI beta cDNA (MS4A2 Human)

Expression

Bacterial

Available Since

June 7, 2005

Availability

Academic Institutions and Nonprofits only
3xFKBP-myc

Plasmid

#20213

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

3xFKBP (FKBP1A Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pGEX Syk(NC)-SH2

Plasmid

#46521

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Syk(NC) (SYK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 7-263

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pRVGP-BB

Plasmid

#15380

Depositor

Stephen Smale

Article

Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

Insert

mU6-BRG/BRM shRNA (Smarca4, Smarca2 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

April 18, 2008

Availability

Academic Institutions and Nonprofits only
pFlox_N-RPB1_BSD_FKBPF36V_EYFP

Plasmid

#163884

Purpose

Acute Depletion of RBP1-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Rbp1 (Polr2a Mouse)

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3GLB1-1/1

Plasmid

#91346

Purpose

Protein expression and purification of human SH3 domain construct SH3GLB1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3GLB1-1/1 (SH3GLB1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-SMAD3

Plasmid

#79507

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human SMAD3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

SMAD3 (SMAD3 Human)

Use

Multisite gateway entry clone

Tags

FLAG

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-MPHOSPH8 ts3

Plasmid

#115876

Purpose

MPHOSPH8 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

MPHOSPH8 shRNA (MPHOSPH8 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAPM-D4 miR30-PPHLN1 ts1

Plasmid

#115877

Purpose

PPHLN1 knockdown

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

PPHLN1 shRNA (PPHLN1 Human)

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+MT-hToca-1-MGD

Plasmid

#33033

Depositor

Marc Kirschner

Article

Ho et al Cell. 2004 Jul 23;118(2):203-16.

Insert

Toca-1 (FNBP1L Human)

Tags

6xMyc

Expression

Mammalian

Mutation

MGD = amino acid substitutions M383I, G384S and D…

Promoter

simian CMV IE94

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pGS_N_3xFLAG

Plasmid

#101654

Purpose

Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

Depositor

Monika Golas, Bjoern Sander

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, 3xFLAG tag

Tags

3xFLAG tag

Expression

Insect

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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ShadowG-SUMO1

Plasmid

#175239

Purpose

Bacterial expression and purification, conjugatable SUMO for SUMOylation

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

SUMO1 (SUMO1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRPL28(K55M) SpHIS5 (pNTI666)

Plasmid

#115436

Purpose

Expresses K55M RPL28 from HIS3 yeast plasmid

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
HIS5

Expression

Yeast

Mutation

K55M; MfeI site in intron

Promoter

Ashbya gospii TEF1 and endogenous

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag-MBD1 R44Q-14-mVenusC

Plasmid

#191394

Purpose

R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

pcDNA3 Flag-MBD1 R44Q-14-mVenusC

Tags

3xFLAG

Expression

Mammalian

Mutation

R44Q

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221-PGK.Pro

Plasmid

#79489

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human PGK promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

PGK Promoter (PGK1 Synthetic, Human)

Use

Multisite gateway entry clone

Promoter

PGK

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-D-TOPO-kcnn4

Plasmid

#164960

Purpose

Zebrafish kcnn4 gene CDS

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnn4 (kcnn4 Zebrafish)

Use

Pcr cloning vector

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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