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We narrowed to 63,690 results for: cloning

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Showing: 8141 - 8160 of 63690 results

  4. qTAG-AAVS1-PGK-Puro

    Plasmid
    #227269
    Purpose
    Empty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Expression
    Mammalian
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227269-plasmid-map-sequence-id-452020

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  6. pCWX-R4DESTR2-PGK-PURO

    Plasmid
    #114319
    Purpose
    Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Puromycin resistance
    Depositor
    Patrick Salmon
    Type
    Empty backbone
    Use
    Lentiviral
    Available since
    Sept. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    114319-plasmid-map-sequence-id-220088

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  7. mEos3.2-MYR-N-5

    Plasmid
    #62769
    Purpose
    Localization: Cytoskeleton , N1 Cloning Vector, Excitation:507 / 572, Emission:516 / 580
    Depositor
    Michael Davidson
    Insert
    MYR
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. PS-CFP2-MYR-N-5

    Plasmid
    #62795
    Purpose
    Localization: Cytoskeleton, N1 Cloning Vector, Excitation:400 / 490, Emission:468 / 511
    Depositor
    Michael Davidson
    Insert
    MYR
    Tags
    PS-CFP
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62795-plasmid-map-sequence-id-210800

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  9. mTurquoise-Talin-C-18

    Plasmid
    #62788
    Purpose
    Localization: Talin/Focal Adhesions, C1 Cloning Vector, Excitation:434, Emission:474
    Depositor
    Michael Davidson
    Insert
    Talin
    Tags
    mTurquoise
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62788-plasmid-map-sequence-id-241035

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  10. mEos2-MYR-N-5

    Plasmid
    #62768
    Purpose
    Localization: Cytoskeleton , N1 Cloning Vector, Excitation:505 / 569, Emission:516 / 581
    Depositor
    Michael Davidson
    Insert
    MYR
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. mEmerlad-MCPB2-C-18

    Plasmid
    #62767
    Purpose
    Localization: Actin Capping Protein , C1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    MCPB2
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    62767-plasmid-map-sequence-id-235388

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  13. pYSD061

    Plasmid
    #212915
    Purpose
    Encodes the S. cerevisiae SUC2 (Invertase) protein (minus signal peptide) as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Publication
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    SUC2 (SUC2 Budding Yeast)
    Expression
    Bacterial
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212915-plasmid-map-sequence-id-421750

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  15. pYSD153

    Plasmid
    #212929
    Purpose
    Encodes the mRuby2 fluorescent protein (Part3b') with the 111AA SCW4 TFP (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Publication
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    mRuby2
    Expression
    Yeast
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212929-plasmid-map-sequence-id-421717

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  16. pYSD141

    Plasmid
    #212928
    Purpose
    Encodes the mRuby2 fluorescent protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.
    Depositor
    Paul Young
    Publication
    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102
    Insert
    mRuby2
    Expression
    Yeast
    Available since
    Feb. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    212928-plasmid-map-sequence-id-421715

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  18. KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRAB

    Plasmid
    #121830
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    x-dCas9(3.7)/KRAB (open)
    Use
    Gateway Cloning and Synthetic Biology
    Tags
    NLS
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121830-plasmid-map-sequence-id-237384

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  20. IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS

    Plasmid
    #121821
    Purpose
    pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sa-dCas9 (open)
    Use
    Gateway Cloning and Synthetic Biology
    Tags
    NLS
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121821-plasmid-map-sequence-id-237374

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Showing: 8141 - 8160 of 63690 results