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pHR_Gal4UAS_pGK_mCherry

Plasmid

#79124

Purpose

Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors (no IRES)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-puro SV40 LT

Plasmid

#13970

Depositor

Thomas Roberts

Article

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

SV40 LT (SV40gp6 )

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pBRC1

Plasmid

#183064

Purpose

Base editing plasmid for Pseudomonas chlororaphis

Depositor

Xuehong Zhang

Article

Yue et al Microb Biotechnol. 2022 Sep;15(9):2324-2

Insert

eSpCas9ppD10A

Use

CRISPR and Synthetic Biology

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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p2lox-cAMPr

Plasmid

#99143

Purpose

Expresses cAMPr in mammalian cells

Depositor

Justin Blau

Article

Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

Insert

cAMPr

Use

Cre/Lox

Tags

cpGFP

Expression

Mammalian

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET-51b-HaloTag11

Plasmid

#175519

Purpose

Expression of HaloTag11 in bacterial cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag11

Tags

His

Expression

Bacterial

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_5x Gal4 UAS

Plasmid

#79119

Purpose

Base vector for cloning Gal4DBDVP64 inducible genes

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-GroEL/ES

Plasmid

#83922

Purpose

Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Gro EL/ES

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pGT-B1

Plasmid

#122579

Purpose

MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-S2

Plasmid

#122583

Purpose

MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pGT-B2

Plasmid

#122580

Purpose

MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pHBJT023

Plasmid

#225174

Purpose

Low copy cloning vector with same multiple cloning site as pUC19 and pSU19 (SmR).

Depositor

Jenny-Lee Thomassin

Article

Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pPEgRNA

Plasmid

#172716

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Article

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

gRNA

Expression

Bacterial

Available Since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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p4xEGFP-N1

Plasmid

#122164

Purpose

Expression of 4xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

4xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p2xEGFP-N1

Plasmid

#122162

Purpose

Expression of 2xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

2xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p8xEGFP-N1

Plasmid

#122168

Purpose

Expression of 8xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

8xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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synIRES_RC25

Plasmid

#189970

Purpose

Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

synIRES_RC25

Use

Cloning

Promoter

N/A

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-mCherry (527)

Plasmid

#70719

Purpose

Expression vector for mCherry purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

mCherry

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGT-S1

Plasmid

#122582

Purpose

MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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RepX_pET28a(+)

Plasmid

#172199

Purpose

Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

Depositor

Taekjip Ha

Article

Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

Insert

RepX superhelicase

Tags

6xHis tag

Expression

Bacterial

Mutation

C18L, C43S, C167V, S400C, C612A

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ131A

Plasmid

#69273

Purpose

Golden Gate entry vector; 1st gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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