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pTH364-SUP45-Y410F

Plasmid

#29395

Depositor

Tobias von der Haar

Article

Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

Insert

SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Y410F mutant gene including genomic sequence 1432…

Promoter

Available since

May 5, 2011

Availability

Academic Institutions and Nonprofits only
pC13N-dCas9-BFP-KRAB

Plasmid

#127968

Purpose

constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-dCas9-NLS-BFP-KRAB

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pNTI647 dCas9-Mxi1 TetR KanMX

Plasmid

#139474

Purpose

Integrates CRISPRi effector and tet-responsive regulator

Depositor

Nicholas Ingolia

Article

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Inserts

dCas9-Mxi1
Tet Repressor

Use

CRISPR

Tags

Mxi1 and NLS

Expression

Yeast

Mutation

D10A, H840A

Promoter

pGPM1 and pTEF1

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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SID4x-dCas9-KRAB

Plasmid

#106399

Purpose

Expresses catalytically inactive dCas9 with N-terminal fusion of Sin3a Interacting Domain (SID) and C-terminal fusion of repressive KRAB domain

Depositor

Jason Gertz

Article

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Inserts

SID4x (MXD1 Human)
KRAB (ZNF10 Human)

Use

Tags

FLAG, HA, and NLS

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFlag IFIT2

Plasmid

#53555

Purpose

mammalian expression of IFIT2

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT2 (IFIT2 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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human CD40 promoter

Plasmid

#53433

Purpose

promoter activity study

Depositor

Etty Benveniste

Article

Nguyen et al J Biol Chem. 2002 Apr 19;277(16):13796-8

Insert

CD40 promoter (CD40 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

CD40

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a(+) 6xHis-TEV-IFIT2

Plasmid

#53558

Purpose

bacterial expression of IFIT2

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT2 (IFIT2 Human)

Use

Tags

6xHIS and TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
EGFP-Caveolin3DGV

Plasmid

#67886

Purpose

Fluorescently tagged Caveolin-3 truncation mutant for expression in mammalian cells

Depositor

Rob Parton

Article

Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

Insert

Cav3 (Cav3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the first 53 amino acids of Caveolin3

Promoter

CMV

Available since

Aug. 19, 2015

Availability

Academic Institutions and Nonprofits only
FUS-9YS-miRFP670-TRF1

Plasmid

#227381

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric FUS IDR, 9YS (TERF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

9YS

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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FUS-27YS-miRFP670-TRF1

Plasmid

#227383

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Article

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric FUS IDR, 27YS (TERF1 Human)

Use

Lentiviral

Tags

Expression

Mutation

27YS

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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