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TRE3GV-somBiPOLES-mKate2

Plasmid

#192579

Purpose

AAV-vector for bidirectional optogenetic manipulation of tTA-positive neurons

Depositor

Simon Wiegert

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

TRE3GV

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR221 Cas9-POLD3

Plasmid

#183197

Purpose

Entry clone

Depositor

Emma Haapaniemi

Publication

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-POLD3

Use

CRISPR and Gateway Cloning

Mutation

n/a

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pOpen-Tlipol (exo-)

Plasmid

#165523

Purpose

DNA polymerase; lacks 3' to 5' exonuclease activity.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Tli DNA polymerase (exo-)

Use

Synthetic Biology

Available since

Aug. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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P63-sp64 Poly A

Plasmid

#59435

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Publication

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 63

Use

Makes synthetic transcripts that contains poly a

Tags

none

Mutation

none

Promoter

SP6

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
P29-sp64 Poly A

Plasmid

#59434

Purpose

synthetic poly A transcripts of VZV

Depositor

Randall Cohrs

Publication

Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

Insert

VZV ORF 29

Use

Makes synthetic transcripts that contains poly a

Tags

none

Mutation

none

Promoter

SP6

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
apoeb (apolipoprotein Eb)_L (OZ529)

Plasmid

#27206

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting apoeb (apoeb Zebrafish)

Use

Zebrafish targeting

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
apoeb (apolipoprotein Eb)_R (OZ530)

Plasmid

#27207

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting apoeb (apoeb Zebrafish)

Use

Zebrafish targeting

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

Plasmid

#218915

Purpose

PiggyBac transposition

Depositor

Sven Eyckerman

Publication

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

V5-TurboID-MUTT2A-SARS-CoV2-NSP7/BlastR (ORF1ab )

Expression

Mammalian

Mutation

2363G>C and 4330T>C

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyA

Plasmid

#218914

Purpose

PiggyBac transposition

Depositor

Sven Eyckerman

Publication

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

V5-TurboID-T2A-SARS-CoV2-NSP7/BlastR (ORF1ab )

Expression

Mammalian

Mutation

2363G>C and 4330T>C

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

Plasmid

#218913

Purpose

PiggyBac transposition

Depositor

Sven Eyckerman

Publication

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

V5-TurboID-MUTT2A-SARS-CoV2-NCAP/BlastR (N )

Expression

Mammalian

Mutation

2363G>C and 5335T>C

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyA

Plasmid

#218912

Purpose

PiggyBac transposition

Depositor

Sven Eyckerman

Publication

Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

Insert

V5-TurboID-T2A-SARS-CoV2-NCAP/BlastR (N )

Expression

Mammalian

Mutation

2363G>C and 5335T>C

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA

Plasmid

#177576

Purpose

Expresses SOUL in neuronal cell types

Depositor

Guoping Feng

Publication

Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

Insert

SOUL

Use

AAV

Tags

tdTomato

Promoter

hSyn

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC19-TgKI-MCS-p2A-eGFP-MCS-polyA

Plasmid

#174028

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Publication

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-eGFP

Use

Zebrafish knock-in tagging

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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MMLV-CAG-G_oT7pol-IRES-BSD

Plasmid

#172369

Purpose

Generation of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin

Depositor

Yoav Ben-Simon, Peter Jonas

Publication

Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

Insert

oT7pol + BSD

Use

Retroviral

Expression

Mammalian

Promoter

CAG

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-Jarid2-polyA

Plasmid

#223811

Purpose

IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.

Depositor

Azusa Inoue

Publication

Matsuwaka et al Nat Cell Biol. 2025 Jan;27(1):28-38. doi

Insert

Jarid2 (Jarid2 Mouse)

Use

In vitro transcription

Tags

Flag

Expression

Mammalian

Promoter

CMV, T7

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-CHIKV-Structural polyprotein

Plasmid

#215698

Purpose

Produces the Chikungunya virus structural polyprotein Capsid-E3-E2-6K-E1

Depositor

Melinda Brindley

Publication

Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

Insert

CHIKV structural polyprotein Capsid-E3-E2-6K-E1

Expression

Mammalian

Promoter

CMV

Available since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA)

Plasmid

#195552

Purpose

Expresses Thbs1 under control of short GFAP promoter

Depositor

Kevin Park

Publication

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

short GFAP

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Nuc-mTurq2-Apollo-NADP+

Plasmid

#193302

Purpose

Expresses mTurq2-Apollo-NADP+ sensor localized to the nucleus

Depositor

Jonathan Rocheleau

Publication

Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acss

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

G6PD (G6PD Human)

Tags

SV40 NLS and mTurqoise2

Expression

Mammalian

Mutation

S40A, R72Q, H201N, K205T

Promoter

T7

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-somBiPOLES-eGFP

Plasmid

#192584

Purpose

AAV-vector for bidirectional optogenetic manipulation of Cre-positive neurons

Depositor

Simon Wiegert

Insert

somBiPOLES

Use

AAV

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Promoter

human synapsin

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLD1

Plasmid

#160805

Purpose

Express POLD1

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLD1 (POLD1 Human)

Use

Retroviral

Mutation

Codon optimized C-terminal 230 amino acids

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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