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pCon-IFl_Con-IFr

Plasmid

#192209

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-F Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IEr

Plasmid

#192210

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-E Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-ICr

Plasmid

#192211

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-C Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IIAr

Plasmid

#192212

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus II-A Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IEl_Con-IEr

Plasmid

#192213

Purpose

Consensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-E Leader and Consensus I-E Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-ICl_Con-ICr

Plasmid

#192214

Purpose

Consensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-C Leader and Consensus I-C Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IIAl_Con-IIAr

Plasmid

#192215

Purpose

Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus II-A Leader and Consensus II-A Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX1-IFl_Con-IFr

Plasmid

#192216

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X1)

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX2-IFl_Con-IFr

Plasmid

#192217

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X2)

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PiggyBac-mProser1-FLAG-IRES-Blast

Plasmid

#226173

Purpose

Expresses full length mouse PROSER1 with 2X C-terminal FLAG tags

Depositor

Kasper Rasmussen

Article

Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

Insert

Proline Serine Rich protein 1 (Proser1 Mouse)

Tags

2X FLAG

Expression

Mammalian

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEMS2075

Plasmid

#158098

Purpose

High Copy number plasmid containing smCBA insert.

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

smCBA

Promoter

smCBA

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pPBbsr2-NRG1-ScNeo

Plasmid

#209911

Purpose

To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

NRG1-ScNeo (NRG1 Human)

Tags

mNeonGreen and mScarlet

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-HBEGF-mNeonGreen

Plasmid

#209912

Purpose

To monitor the expression of HB-EGF, the plasmid encodes a recombinant HB-EGF fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

HBEGF-mNeonGreen (HBEGF Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-EREG-mNeonGreen

Plasmid

#209914

Purpose

To monitor the expression of Epiregulin, the plasmid encodes a recombinant Epiregulin fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

EREG-mNeonGreen (EREG Human)

Use

Lentiviral

Tags

mNeonGreen

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbleo-TGFa-ScNeo

Plasmid

#209904

Purpose

To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-ScNeo (TGFA Human)

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-TGFa-ScNeo

Plasmid

#209908

Purpose

To monitor the status of TGFα, the plasmid encodes a recombinant TGFα, fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-ScNeo (TGFA Human)

Tags

GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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(attB) tdMCP-tagged BRaf

Plasmid

#226165

Purpose

Used to integrate 3xFlag-tdMCP-BRaf without MS2-circRNA barcdes into landing pad cell lines containing a single promoter.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

3xFlag-tdMCP-BRaf (BRAF Human)

Use

Bxb1 attb, no promoter

Tags

3xFlag-tdMCP and IRES-mCherry-P2A-PuroR

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST17-DXO

Plasmid

#177993

Purpose

For purification of N-terminally His-tagged DXO from bacteria

Depositor

Karla Feijs

Article

Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

Insert

DXO (DXO Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCHMP3-NS3-Blue

Plasmid

#223533

Purpose

Mammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing the NS3 cleavage site, along with a blue fluorescent protein.

Depositor

Winfried Weissenhorn

Article

Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

Insert

CHMP3 (CHMP3 Human)

Tags

NS3 Hepatitis C protease and mTurquoise

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCHMP3-mut-NS3-Green

Plasmid

#223530

Purpose

Mammalian expression of CHMP3 fused to the hepatitis C virus protease NS3 through a short linker containing mutated NS3 cleavage site, along with a green fluorescent protein.

Depositor

Winfried Weissenhorn

Article

Wang et al Viruses. 2023 Nov 22;15(12):2289. doi: 1

Insert

CHMP3 (CHMP3 Human)

Tags

NS3 Hepatitis C protease and mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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