Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 15,557 results for: LEA

Showing: 8181 - 8200 of 15557 results

  1. pOP5GP

    Plasmid
    #112610
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-GST and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    112610-plasmid-map-sequence-id-220240

    Enlarge

  2. pLenti.CAG.H2B-cerFP.W.SINloxP

    Plasmid
    #51006
    Purpose
    Lentivector that expresses H2B-cerFP from internal CAG enhancer-promoter
    Depositor
    Rusty Lansford
    Insert
    H2B-cerFP
    Use
    Lentiviral
    Tags
    histone 2B
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51006-plasmid-map-sequence-id-77912

    Enlarge

  3. pOpen-ECOpolA

    Plasmid
    #165532
    Purpose
    DNA polymerase that also exhibits 3'-5' and 5'-3' exonuclease activity.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    E. coli DNA Polymerase I
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165532-plasmid-map-sequence-id-339121

    Enlarge

  4. pOP5GT

    Plasmid
    #112611
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GST tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-GST and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112611-plasmid-map-sequence-id-220247

    Enlarge

  5. pOP5MP

    Plasmid
    #112612
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and MBP tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-MBP and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112612-plasmid-map-sequence-id-220250

    Enlarge

  6. pOP5BT

    Plasmid
    #112609
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-GB1 and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112609-plasmid-map-sequence-id-220239

    Enlarge

  7. FusionRed-FTR940-N-11

    Plasmid
    #62737
    Purpose
    Localization: Nuclear - Nucleolus, N1 Cloning Vector, Excitation:580, Emission:608
    Depositor
    Michael Davidson
    Insert
    FTR940
    Use
    Tags
    FusionRed
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pET15b (I-WcaINhis) K226I

    Plasmid
    #195620
    Purpose
    Expresses I-WcaI homing endonuclease from Wickerhamomyces canadensis containing the K226I mutation
    Depositor
    Frederick Gimble
    Insert
    I-WcaI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A1930T, A1931T
    Promoter
    T7
    Available since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    195620-plasmid-map-sequence-id-384568

    Enlarge

  9. pET15b (I-WcaINhis) K125I

    Plasmid
    #195619
    Purpose
    Expresses I-WcaI homing endonuclease from Wickerhamomyces canadensis containing the K125I mutation
    Depositor
    Frederick Gimble
    Insert
    I-WcaI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A2030T, A2031T
    Promoter
    T7
    Available since
    Feb. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    195619-plasmid-map-sequence-id-384567

    Enlarge

  10. pOP5TT

    Plasmid
    #112613
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-thioredoxin and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112613-plasmid-map-sequence-id-220251

    Enlarge

  11. pOP5BP

    Plasmid
    #112608
    Purpose
    Used for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Use
    Tags
    8His-GB1 and Avi
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    112608-plasmid-map-sequence-id-220237

    Enlarge

  12. HA-CPI-17-wt-NLS

    Plasmid
    #100776
    Purpose
    Mammalian expression of CPI-17 nuclear
    Depositor
    Joseph Gordon
    Insert
    CPI-17-NLS (PPP1R14A Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    100776-plasmid-map-sequence-id-193196

    Enlarge

  13. pOpen-EcoRV

    Plasmid
    #165548
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    EcoRV
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165548-plasmid-map-sequence-id-339428

    Enlarge

  14. pOpen-KTHEAQpol

    Plasmid
    #165542
    Purpose
    Exonuclease-free, N-terminal deletion mutant of Taq DNA polymerase.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    KlenTaq1 polymerase
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165542-plasmid-map-sequence-id-339275

    Enlarge

  15. pOpen-SapIR

    Plasmid
    #165537
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    SapI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165537-plasmid-map-sequence-id-339127

    Enlarge

  16. pOpen-EcoRIR

    Plasmid
    #165504
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    EcoRI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165504-plasmid-map-sequence-id-338511

    Enlarge

  17. pOpen-SfiIM

    Plasmid
    #165538
    Purpose
    Site-specific DNA methyltransferase; protects DNA from cleavage by SfiI.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    M.SfiI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165538-plasmid-map-sequence-id-339128

    Enlarge

  18. pOpen-XbaIR

    Plasmid
    #165567
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    XbaI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 18, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165567-plasmid-map-sequence-id-340099

    Enlarge

  19. pOpen-PstIR

    Plasmid
    #165526
    Purpose
    Restriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    PstI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165526-plasmid-map-sequence-id-339060

    Enlarge

  20. pHS2

    Plasmid
    #81037
    Purpose
    HO endonuclease expression
    Depositor
    John McCusker
    Insert
    HYGMX
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    81037-plasmid-map-sequence-id-274379

    Enlarge

Showing: 8181 - 8200 of 15557 results