We narrowed to 4,210 results for: Pet2

1. PARP1 FL

   Plasmid

   #169815

   Purpose

   Expresses codon optimised full length His tagged PARP1 in bacterial cells

   Depositor

   David Neuhaus

   Article

   Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

   Insert

   Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Human, Synthetic)
   

   Use

   Tags

   MKHHHHHHMKQ

   Expression

   Bacterial

   Mutation

   Valine 762 mutated to an alanine

   Promoter

   T7 promoter

   Available since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHis-BRD4 BD1

   Plasmid

   #196544

   Purpose

   Expression of BRD4 Bromodomain (BD1) in E.coli

   Depositor

   Byung Il Lee

   Article

   Kim et al Sci Rep. 2023 Jul 4;13(1):10805. doi: 10

   Insert

   BRD4 BD1 (BRD4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-(-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis

   Plasmid

   #62373

   Purpose

   Expression of (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)dGFP-9xGGS-Cath-NLS-Cas9-6xHis
   

   Use

   Tags

   (-30)dGFP, 6xHis tag, Cathepsin cleavage site, an…

   Expression

   Bacterial

   Mutation

   Y67S amino acid substitution in (-30)GFP renders …

   Promoter

   T7

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-EIN-AlkBH5(66-292)

   Plasmid

   #125732

   Purpose

   Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.

   Depositor

   Vincenzo Venditti

   Article

   Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

   Insert

   AlkBH5(66-292) (ALKBH5 Human)
   

   Use

   Tags

   6xHis and EIN

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-VPR-S-L1-dCjCas9 MiniCAFE

   Plasmid

   #169910

   Purpose

   Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   MiniCAFE
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D8A, HNH-truncation (Δ495–609 aa) in CjCas9

   Promoter

   CMV

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. proISG15 (2-165)

   Plasmid

   #110762

   Purpose

   Bacterial expression of proISG15

   Depositor

   David Komander

   Article

   Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

   Insert

   ISG15 (ISG15 Human)
   

   Use

   Tags

   N-His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. His6tev CBP BCHZ (1079-1751)

   Plasmid

   #99341

   Purpose

   Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

   Depositor

   Peter Wright

   Article

   Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

   Insert

   mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)
   

   Use

   Tags

   His6tev

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. His6tev CBP HAT (1324-1700)

   Plasmid

   #99340

   Purpose

   bacterial expression of His tagged mouse CBP HAT domain (1324-1700)

   Depositor

   Peter Wright

   Article

   Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

   Insert

   mouse CBP HAT domain (1324-1700) (Crebbp Mouse)
   

   Use

   Tags

   His6tev

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. H6SUMO-IntC-P53(304-393)

   Plasmid

   #200316

   Purpose

   bacterial expression of P53 TET-CTD for segmental labeling

   Depositor

   Peter Wright

   Article

   Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

   Insert

   p53 (304-393) (TP53 Human)
   

   Use

   Tags

   His6-SUMO and designed intein C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. H6GB1tevP53(1-303)IntN

    Plasmid

    #200312

    Purpose

    Bacterial expression of P53 NTAD-DBD for segmental labeling

    Depositor

    Peter Wright

    Article

    Krois et al Biochemistry. 2022 Dec 6;61(23):2709-271

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His6GB1tev and designed intein N

    Expression

    Bacterial

    Mutation

    M133L, V203A, N239Y, N268D

    Promoter

    T7

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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