We narrowed to 14,442 results for: NTS

1. FB002

   Plasmid

   #119676

   Purpose

   Terminator tub from Neurospora crassa domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   Terminator tub
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. FB008

   Plasmid

   #119706

   Purpose

   Terminator trpC from Aspergillus nidulans domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   Terminator trpC
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. KHBD00051

   Plasmid

   #34213

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG10159 (BEAF-32 Fly)
   

   Use

   Gateway donor vector

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3 Flag MKK3b(Glu, delete LXL)

   Plasmid

   #50450

   Purpose

   activates p38 in the signaling pathway

   Depositor

   Roger Davis

   Article

   Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

   Insert

   MKK3b (MAP2K3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted L25-I27, changed Serine 218 to Glutamic A…

   Promoter

   CMV

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

5. KHBD00298

   Plasmid

   #34424

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG4029 (jumu Fly)
   

   Use

   Gateway donor vector

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

6. KHBD00158

   Plasmid

   #34312

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG33183 (Hr46 Fly)
   

   Use

   Gateway donor vector

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

7. Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

   Plasmid

   #64207

   Purpose

   mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

   Inserts

   Cry2 (CRY2 Mustard Weed)
   RGS4(del 1-33) (RGS4 Human)
   

   Tags

   mcherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

8. BRCA1-(360-498)

   Plasmid

   #35068

   Depositor

   Cheryl Arrowsmith

   Article

   Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

   Insert

   BRCA1 (BRCA1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains aa 498-360

   Promoter

   T7

   Available Since

   April 4, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126777

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   Sniper SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   F539S, M763I, K890N

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221 NLRP1 M77T

    Plasmid

    #166818

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation M77T (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    M77T

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221 NLRP1 A54T

    Plasmid

    #166815

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A54T (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    A54T

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC19-F3UXG6

    Plasmid

    #103108

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    F3UXG6(Cas9 coding gene from Streptococcus sanguinis SK49)
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221 NLRP1 FIIND (F1079M-L1373) S1213A

    Plasmid

    #166832

    Purpose

    Gateway pDONR vector containing NLRP1 FIIND with autoprocessing deficient S1213A mutation (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KHBD00551

    Plasmid

    #39646

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG1849 (run Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

15. BRCA1-(758-1057)

    Plasmid

    #35085

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 758-1057

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pCEP4-myc-HLA-A24-VN

    Plasmid

    #135508

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*24:02

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*24:02 (HLA-A Human)
    

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19-C7TEQ6

    Plasmid

    #103092

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    C7TEQ6(Cas9 coding gene from Lactobacillus rhamnosus (strain ATCC 53103 / GG))
    

    Use

    CRISPR; Cloning vector

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-1 (GB1204)

    Plasmid

    #75405

    Purpose

    Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    AtU6-1 promoter
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. 373_ESR1 in pmiRGlo

    Plasmid

    #78134

    Purpose

    luciferase reporter for miRNA activity

    Depositor

    Heidi Schwarzenbach

    Article

    Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    3'UTR containing miRNA binding sites

    Promoter

    PGK

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. B-SpCas9

    Plasmid

    #126760

    Purpose

    Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    amino acids 1005-1013 replaced with two glycine

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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