We narrowed to 9,514 results for: try

1. pCDF-casBCDE(-12)

   Plasmid

   #89731

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-12) truncated by 12 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a shorter vers…

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDF-casBCDE(-18)

   Plasmid

   #89732

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-18) truncated by 18 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a shorter vers…

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDF-casBCDE(-3)

   Plasmid

   #89729

   Purpose

   Expresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-3) truncated by 3 nucleotides at the leader-distal end.

   Depositor

   Konstantin Kuznedelov, Konstantin Severinov

   Article

   Kuznedelov et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Mutation

   Derivative CRISPR array containing a shorter vers…

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. CSKP_HUMAN_D0

   Plasmid

   #79714

   Purpose

   This plasmid encodes the kinase domain of CSKP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   Markus Seeliger, John Chodera, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   CSKP (CASK Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pEN_TT 3XFLAG-S37A-GATA6-3XAU1

   Plasmid

   #72613

   Purpose

   Gateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged S37A mutant human GATA6

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA binding protein 6 (GATA6 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   3XAU1 and 3XFLAG

   Expression

   Bacterial

   Mutation

   Changed serine37 to alanine

   Promoter

   TRE tight promoter

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV[Exp]-EF1A>Tet3G:IRES:Neo

   Plasmid

   #198756

   Purpose

   Vector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418

   Depositor

   Nael Nadif Kasri

   Article

   Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

   Insert

   Tet3G, IRES, Neo
   

   Use

   Lentiviral

   Promoter

   EF1A

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Epac-S-H187

   Plasmid

   #170348

   Purpose

   mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   mT2Del_EPACdDEPCD_Q270E_tdcp173Ven(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC1-V16-hGDNF

   Plasmid

   #122035

   Purpose

   AAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and hGDNF expression

   Depositor

   Liliane Tenenbaum

   Article

   Chtarto et al Mol Ther Methods Clin Dev. 2016 Mar 30;5

   Insert

   hGDNF (GDNF Human)
   

   Use

   AAV

   Available Since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR207 SARS-CoV-2 NSP3

   Plasmid

   #141257

   Purpose

   Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Has Service

   Cloning Grade DNA

   Insert

   NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pHR-YTHDC1-FL-mCherry-Cry2

    Plasmid

    #177124

    Purpose

    Express Full-length YTHDC1 with mCherry and CRY2 tag for Optodroplet Assay

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Tags

    mCherry-CRY2

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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