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p101_CRISPRai_PiggyBac

Plasmid

#213777

Purpose

Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

VPR-dSaCas9
dSpCas9-KRAB-BFP
zeocin
Tet-On transactivator

Use

CRISPR

Tags

Expression

Mutation

Promoter

EF1a (EF-1-alpha intron a) and Tet_On_TRE

Available since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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LRCherry2.1

Plasmid

#108099

Purpose

Lentiviral expression plasmid of sgRNA with mCherry

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCH67 (crRNA expression vector)

Plasmid

#217337

Purpose

CROP-seq crRNA expression vector with GFP and puro marker

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

AsDR-filler-AsDR8, GFP-puro

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo

Plasmid

#108101

Purpose

Lentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

FLAG tag

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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LRG2.1

Plasmid

#108098

Purpose

Lentiviral expression plasmid of sgRNA with GFP

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter for sgRNA expression and EFS promoter…

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCH49 (crRNA expression vector)

Plasmid

#217338

Purpose

CROP-seq crRNA expression vector with GFP and neo marker

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

AsDR-filler-AsDR8, GFP-neo

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAC150-PBLHL-4xHS-EF1a-DEST

Plasmid

#48234

Purpose

PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Type

Empty backbone

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCambia-PUP-IT

Plasmid

#186478

Purpose

Expresses POI-PUP-IT fusion in Plants

Depositor

Jen Sheen

Article

Ye et al Nature. 2022 Sep 14. pii: 10.1038/s41586

Insert

FLAG-Pup(E)

Use

Tags

Triple Flag Tag

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-Spike

Plasmid

#145031

Purpose

Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

Depositor

Fang Li

Article

Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

Insert

SARS-CoV Spike

Use

Tags

C9 and CD5 signal peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Puro-Cas9 donor

Plasmid

#58409

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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