Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 4,425 results for: Pet2;

Showing: 1231 - 1260 of 4425 results

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pET29b-EPN01(∆L1+∆L2)-His

Plasmid

#84511

Purpose

E.coli version of EPN01(∆L1+∆L2)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN01(∆L1+∆L2)-His

Tags

His tag

Expression

Bacterial

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pET28A-p53 (1-73) (P27A)

Plasmid

#62068

Purpose

Expression of human p53 (1-73) (P27A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P27A

Promoter

T7

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
Axin GID 1-2/pET29b

Plasmid

#16307

Depositor

Peter Klein

Article

Hedgepeth et al Mol Cell Biol. 1999 Oct . 19(10):7147-57

Insert

Axin GID 1-2 (axin1.L Frog)

Tags

His

Expression

Bacterial

Mutation

Amino acids 320-429

Available Since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only
pET23b-proMMP-7-E215Q

Plasmid

#29642

Depositor

William Parks

Article

Ra et al J Biol Chem. 2009 Oct 9. 284(41):27924-3

Insert

proMMP-7 (MMP7 Human)

Expression

Bacterial

Mutation

E215Q Catalytically Inactive

Available Since

April 29, 2011

Availability

Academic Institutions and Nonprofits only
pET28(a)-hFX(I154F)-HA

Plasmid

#14992

Depositor

Michael Ristow

Insert

human frataxin cDNA (FXN Human)

Tags

AU1, HA, and HIS

Expression

Bacterial

Mutation

contains cDNA coding sequence for amino acids 88-…

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pET24a-VHH-TagRFP657

Plasmid

#243767

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to TagRFP657. VHH-TagRFP657 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and TagRFP657-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_F113L

Plasmid

#248016

Purpose

Expression of synthetic Kemp eliminase (Des27.7 F113L)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_F113L

Tags

12xHis-bdSumo

Expression

Bacterial

Mutation

F113L

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_noCoreStab

Plasmid

#248014

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS&ActiveSite stability)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_noCoreStab

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_PROSS

Plasmid

#248012

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_PROSS

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7_ModularAssembly

Plasmid

#248011

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7. ModularAssembly)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27

Plasmid

#247996

Purpose

Expression of synthetic Kemp eliminase (Des27)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.3

Plasmid

#247999

Purpose

Expression of synthetic Kemp eliminase (Des27.3)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.3

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.10

Plasmid

#248005

Purpose

Expression of synthetic Kemp eliminase (Des27.10)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.10

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.1

Plasmid

#247997

Purpose

Expression of synthetic Kemp eliminase (Des27.1)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.1

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.4

Plasmid

#248000

Purpose

Expression of synthetic Kemp eliminase (Des27.4)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.4

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.12

Plasmid

#248007

Purpose

Expression of synthetic Kemp eliminase (Des27.12)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.12

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des61.1

Plasmid

#248010

Purpose

Expression of synthetic Kemp eliminase (Des61.1)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des61.1

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.5

Plasmid

#248001

Purpose

Expression of synthetic Kemp eliminase (Des27.5)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.5

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des61

Plasmid

#248009

Purpose

Expression of synthetic Kemp eliminase (Des61)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des61

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.2

Plasmid

#247998

Purpose

Expression of synthetic Kemp eliminase (Des27.2)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.2

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.6

Plasmid

#248002

Purpose

Expression of synthetic Kemp eliminase (Des27.6)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.6

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.11

Plasmid

#248006

Purpose

Expression of synthetic Kemp eliminase (Des27.11)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.11

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7

Plasmid

#248003

Purpose

Expression of synthetic Kemp eliminase (Des27.7)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.9

Plasmid

#248004

Purpose

Expression of synthetic Kemp eliminase (Des27.9)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.9

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.13

Plasmid

#248008

Purpose

Expression of synthetic Kemp eliminase (Des27.13)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.13

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-VHH-mNeptune2.5

Plasmid

#243765

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mNeptune2.5. VHH-mNeptune2.5 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mNeptune2.5-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-His6-LONP1

Plasmid

#247937

Purpose

Expression of human LONP1 with methionine 115 as its first residue (mature mitochondrial-matrix localized form)

Depositor

Gabriel Lander

Article

Shin et al Nat Commun. 2021 May 28;12(1):3239. doi:

Insert

LONP1 (LONP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

encodes aa 115-959

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-VHH-mCardinal2

Plasmid

#243766

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCardinal2. VHH-mCardinal2 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mCardinal2-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-VHH-iRFP670

Plasmid

#243768

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to iRFP670. VHH-iRFP670 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and iRFP670-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_SpyTag003-L-IGHJ4_2-His

Plasmid

#233335

Purpose

To express nanobody IGHJ4_2 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ4_2-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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