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pET12a-AL-09 FL Q42K C214S

Plasmid

#47089

Depositor

Marina Ramirez-Alvarado

Publication

Levinson et al Sci Rep. 2013;3:1278. doi: 10.1038/srep0

Insert

AL-09 FL Q42K C214S (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

Q42K C214S

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET12a-AL-09 FL I34N C214S

Plasmid

#47088

Depositor

Marina Ramirez-Alvarado

Publication

Levinson et al Sci Rep. 2013;3:1278. doi: 10.1038/srep0

Insert

AL-09 FL I34N C214S (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Mutation

I34N C214S

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
γA3/N-GFP

Plasmid

#33113

Depositor

Greg Phillips

Publication

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Cytoplasmic domain has been replaced with the N-c…

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pT.cruzi- 60sP0-Kn107_6xHis

Plasmid

#256087

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

60S acidic ribosomal protein P0, putative

Tags

6xHis-T7Tag-XpressTag

Expression

Bacterial

Promoter

T7

Available since

May 28, 2026

Availability

Academic Institutions and Nonprofits only

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pT.cruzi-40sS21-hypo_6xHis

Plasmid

#256078

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

40S ribosomal protein S21,hypothetical protein

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pT.cruzi-hypo-CBP_6xHis

Plasmid

#256082

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Hypothetical protein,calcium-binding protein

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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pT.cruzi-Tc2-TolT_6xHis

Plasmid

#256084

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Surface protein TolT, putative

Tags

TrxA-6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO-Rabep1 shRNA 369958-CMV-delta5_2 Rabep1

Plasmid

#254778

Purpose

Rabep1 shRNA 369958 with expression of Rabep1 trucation lacking rab5 binding domain in pLKO backbone

Depositor

Qing Deng

Publication

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

shRNA 369958 and truncated Rabep1 (RABEP1 Human)

Use

Lentiviral

Mutation

5/2 deletion

Promoter

CMV

Available since

May 14, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-GFP-pa-u6ac-Rabep1-1-guides

Plasmid

#254761

Purpose

Rabep1 gRNAs for neutrophil specific KO in tol2 backbone

Depositor

Qing Deng

Publication

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Rabep1 (rabep1 Zebrafish)

Use

CRISPR; Zebrafish expression

Promoter

Lyzc

Available since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-DNAJC13(ylt1)

Plasmid

#246662

Purpose

Mammalian expression of mutant, eGFP tagged DNAJC13

Depositor

Braden Lobingier

Publication

Adoff et al Mol Biol Cell. 2025 Sep 1;36(9):ar114. d

Insert

DNAJC13 (DNAJC13 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Mutated 2206-Y 2207-L 2208-T to Alanines

Promoter

CMV

Available since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-DNAJC13(hpd)

Plasmid

#246663

Purpose

Mammalian expression of DnaJ domain mutant, eGFP tagged DNAJC13

Depositor

Braden Lobingier

Publication

Adoff et al Mol Biol Cell. 2025 Sep 1;36(9):ar114. d

Insert

DNAJC13 (DNAJC13 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Mutated 1332-H, 1333-P, 1334-D to Alanines

Promoter

CMV

Available since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-mGL-bGH

Plasmid

#235255

Purpose

mGreenLantern expression to serve as a transfection marker, EFS-driven

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mGreenLantern

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF4x1-mRuby2-bGH

Plasmid

#235274

Purpose

3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-mRuby2-bGH

Plasmid

#235253

Purpose

ComMAND base gene regulating mRuby2, EFS-driven

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF6x1-mRuby2-bGH

Plasmid

#235275

Purpose

3' microRNA target site expression for dual-vector iFFL circuit (open-loop)

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2-TS.FF4x1-bGH

Plasmid

#235272

Purpose

3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2-TS.FF6x1-bGH

Plasmid

#235273

Purpose

3' microRNA target site expression for dual-vector iFFL circuit (open-loop)

Depositor

Kate Galloway

Publication

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_W208L

Plasmid

#221486

Purpose

NKX2-1 W208L DNA binding mutation lentiviral overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

W208L

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223_NKX2-1_DelCTerm_1-223AA

Plasmid

#221476

Purpose

Gateway cloning of NKX2-1 C-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Mutation

Deletion of 224-371AA C-terminal domain

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_DelNTerm_160-371AA

Plasmid

#221475

Purpose

Gateway cloning of NKX2-1 N-terminal truncation mutation overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway Cloning

Mutation

Deletion of 1-159AA N-terminal domain

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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