We narrowed to 62,186 results for: cloning
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Plasmid#119714PurposeTerminator paf from P. chrysogenum domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformationDepositorInsertTerminator paf
UseSynthetic Biology; Domestication of dna parts for…Available SinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-strepII-3C-LIC-Amp
Plasmid#108707PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_Puro
Plasmid#214680PurposeLentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
plix403-V5-N-mECFP
Plasmid#223954PurposeGateway cloning of genes of interest with an N-terminal V5-mECFP fusion; generation of stable cells with doxycycline inducible expressionDepositorTypeEmpty backboneUseLentiviralTagsV5-mECFPAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
p4X-UASTattB
Plasmid#189858PurposeFly cloning and transformationDepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZMU-Dest
Plasmid#105089PurposeYeast expression vector for Gateway cloning and SUS bridge assaysDepositorTypeEmpty backboneTagsmycExpressionYeastAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
B52 + SMARCAL1 sgSTOP
Plasmid#100715PurposeB52 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)DepositorInsertsgSTOP targeting SMARCAL1 (cloned using BbsI)
UseCRISPRExpressionMammalianPromoterU6 promotersAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXTK-BB1 [Backbone_RK2-KanR_PartTypeBB]
Plasmid#229387PurposeXanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR_PartTypeBB and an sfGFP dropoutDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXTK-BB2 [Backbone_RK2-KanR-TetR_PartTypeBB]
Plasmid#229388PurposeXanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR-TetR_PartTypeBB and an sfGFP dropoutDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pMC-Ura-Cen-lvl1
Plasmid#176182Purposepreassembled yeast MoClo level-1 backbone with GFP dropout and URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
IX001: pBS/Fiber
Plasmid#131754PurposePlasmid containing Ad5 Fiber gene flanked by homology arms for insertion into the recombinant Ad5 vector IQ013 to generate tropism modified vectors.DepositorInsertAd5 wild-type Fiber
UseSynthetic BiologyPromoterN/AAvailable SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
JN402: pMVP (R4-R3) Firefly Luciferase
Plasmid#121741PurposepMVP R4-R3 entry plasmid, contains Firefly Luciferase for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertFirefly Luciferase
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KJ201: pMVP (R4-R3) osTir1 open
Plasmid#121743PurposepMVP R4-R3 entry plasmid, contains osTir1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertosTir1 (open)
UseGateway Cloning and Synthetic BiologyTags9x myc epitope tagAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IG401: pMVP (L1-R5) RIP
Plasmid#121694PurposepMVP L1-R5 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assemblyDepositorInsertRIP
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HE401: pMVP (L1-R5) shRNA
Plasmid#121698PurposepMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assemblyDepositorTypeEmpty backboneUseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HB501: pMVP (L1-L4) RIP
Plasmid#121688PurposepMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assemblyDepositorInsertRat Ins1 promoter (RIP; β-cell specific)
UseGateway Cloning and Synthetic BiologyAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB1_34_RPS25Att
Plasmid#98526PurposeBB1 with insert: RPS25AttDepositorInsertRPS25Att
ExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p5E-Ui4-eSIBR
Plasmid#82586Purpose5'entry vector containing human ubiquitin C promoter followed by intronically-expressed eSIBR cassette for ubiquitous expression, RNAiDepositorInserthuman ubiquitin C promoter followed by intronically expressed eSIBR cassette
PromoterNoneAvailable SinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
p3E-YFP-HA no-pA
Plasmid#82590Purpose3' entry vector containing HA epitope fused to C-terminus of YFP without pA for imaging, epitope labeling/purificationDepositorInsertYFP-HA (no pA)
PromoterNoneAvailable SinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only