We narrowed to 16,981 results for: tes

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mHDAC7-Nterm (23-504) pEF6-V5

Plasmid

#73270

Purpose

Expression of mouse HDAC7-Nterm in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Tags

His/V5

Expression

Mammalian

Mutation

deleted amino acids 505-938

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCR4-Dact1(1250-1601)

Plasmid

#52046

Purpose

To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.

Depositor

Ben Cheyette

Article

Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.

Insert

Dact1(1250-1601) (Dact1 Mouse)

Expression

Bacterial

Available Since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
pECE-M2-BAIAP2 S366A

Plasmid

#31657

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 S366A (BAIAP2 Human)

Tags

M2

Expression

Mammalian

Mutation

changed Serine 366 to Alanine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-4SA(S164)

Plasmid

#33083

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2010 Jan 1. 24(1):72-85.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S61A, S109A, S127A, S381A

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pRLCon1-1746

Plasmid

#31446

Depositor

Gerhart Ryffel

Article

Wirsing et al PLoS One. 2011;6(11):e27438. Epub 2011 N

Insert

HNF4A 3'UTR (short) (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pGEX-KG/Net1

Plasmid

#28260

Depositor

Jeffrey Frost

Article

Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

Insert

Net1 (Net1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

April 11, 2011

Availability

Academic Institutions and Nonprofits only
pIS1 IMP-1 rc

Plasmid

#21643

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCSP1-mp53AS

Plasmid

#20896

Depositor

Stefano Piccolo

Article

Cordenonsi et al Cell. 2003 May 2. 113(3):301-14.

Insert

p53AS (Trp53 Mouse)

Expression

Mammalian

Mutation

5' natural EcoRV and SacI restriction sites …

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only
ZE38 pCS2P+ sp5l ORF

Plasmid

#17079

Depositor

Randall Moon

Article

Thorpe et al Development. 2005 Apr. 132(8):1763-72.

Insert

sp5l ORF (sp5l Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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L1-1151Y>A

Plasmid

#13269

Depositor

Vance Lemmon

Article

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Expression

Bacterial

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pAG225 HIPK1 3'UTR mut

Plasmid

#12044

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

HIPK1 3'UTR mut (HIPK1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE

Plasmid

#236241

Purpose

Lentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmerald

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

CaV1.2 (CACNA1C Rabbit)

Use

Lentiviral

Tags

mEmerald

Promoter

human Synapsin 1

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mScarlet-CAAX2 WPRE

Plasmid

#236232

Purpose

AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mScarletI

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLViN-iRFP670-α-cateninWT

Plasmid

#229702

Purpose

Lentiviral expression of iRFP670-alpha-cateninWT in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

CTNNA1 (alpha-catenin) Human (CTNNA1 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Puro-PNPT1 S484A

Plasmid

#223316

Purpose

Lentiviral vector expressing human PNPT1 S484A variant

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed serine 484 to alanine

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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TRIPZ-bioGEF-SUMO2nc-PURO

Plasmid

#208043

Purpose

Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

SUMO2nc (SUMO2 Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Mutation

Q90P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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kiCAP-AAV-MDV1A

Plasmid

#196679

Purpose

Rep/Cap plasmid for the production of MDV1A, an AAV capsid with CNS tropism in mice.

Depositor

Pardis Sabeti

Article

Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004

Insert

AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589

Use

AAV

Mutation

RSVGSVY insert between amino acids 588 and 589 of…

Promoter

p41

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDest-1.7kbfabp6-eGFP-pA-CrymCherry

Plasmid

#159088

Purpose

LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts

Depositor

John Rawls

Article

Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

Inserts

1.7kb fabp6 promoter (fabp6 Zebrafish)
eGFP
polyA

Use

Fluorescent reporter

Promoter

1.7kb fabp6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)

Plasmid

#160633

Purpose

TU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31-RDF

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-E6AP Hect

Plasmid

#165102

Purpose

bacterial expression of a GST fusion of Human E6AP Hect domain

Depositor

Arthur Haas

Article

Ronchi et al Methods Mol Biol. 2012;832:197-218. doi:

Insert

E6AP Hect (UBE3A Human)

Tags

GST from plasmid.

Expression

Bacterial

Mutation

E6AP Hect domain Amino acids 495-738 (5'QLNP…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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