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pET28c_S(-31)-M30_dT7term

Plasmid

#112253

Purpose

Expresses the S(-31)-M30 apta-FRET RNA origami structure.

Depositor

Ebbe Sloth Andersen

Article

Jepsen et al Nat Commun. 2018 Jan 2;9(1):18. doi: 10.

Insert

S(-31)-M30 apta-FRET RNA origami with T7 promoter and terminators.

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28c_S*5-M5_dT7term

Plasmid

#112254

Purpose

Expresses the S*5-M5 apta-FRET RNA origami structure.

Depositor

Ebbe Sloth Andersen

Article

Jepsen et al Nat Commun. 2018 Jan 2;9(1):18. doi: 10.

Insert

S*5-M5 apta-FRET RNA origami with T7 promoter and terminators.

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-GST-P(11)4

Plasmid

#185789

Purpose

Encodes glutathione S-transferase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

GST-P(11)4

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-HisTim12_CtoA

Plasmid

#170281

Purpose

Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

Insert

yeast Tim12 (TIM12 Budding Yeast)

Tags

TEV-cleavable N-terminal His tag (on backbone)

Expression

Bacterial

Mutation

mutated two non-conserved Cys to Ala

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_hTLN2-head

Plasmid

#164836

Purpose

Expression of human talin2 head

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His8

Expression

Bacterial

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB4

Plasmid

#134688

Purpose

Expresses His6-AmmB4 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB4

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB3

Plasmid

#134687

Purpose

Expresses His6-AmmB3 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB3

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB2

Plasmid

#134686

Purpose

Expresses His6-AmmB2 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB2

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB1

Plasmid

#134685

Purpose

Expresses His6-AmmB1 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB1

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-tglF

Plasmid

#134681

Purpose

Expresses His6-TglF in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglF

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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