We narrowed to 15,736 results for: LEA

1. pGTEVDNIb

   Plasmid

   #122530

   Purpose

   Binary plasmid with a TEV (NIb deleted) clone

   Depositor

   Jose-Antonio Daros

   Article

   Daros et al Methods Mol Biol. 2020;2083:303-312. doi

   Insert

   Tobacco etch virus (NIb deleted) cDNA
   

   Use

   Potyvirus vectors to produce carotenoids in plants

   Available Since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. XClone-Cas9-Cronus-T2A-BFP

   Plasmid

   #141209

   Purpose

   Tunable and temporal expression control of Cas9

   Depositor

   Rory Johnson

   Insert

   Cas9
   

   Expression

   Mammalian

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. RAB3B (3DZ8)

   Plasmid

   #32879

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   RAB3B (RAB3B Human)
   

   Tags

   His tag (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   amino acids 18-190 only

   Available Since

   Jan. 11, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pET22b-EC12WTC9A

   Plasmid

   #47924

   Purpose

   Expresses E-cadherin EC12 WT with C9A mutation in E.Coli.

   Depositor

   Tanja Kortemme

   Article

   Ritterson et al J Am Chem Soc. 2013 Aug 7.

   Insert

   E-Cadherin EC12
   

   Tags

   6xHis and TEV Cleavage

   Expression

   Bacterial

   Mutation

   changed Cysteine 9 to Alanine

   Promoter

   T7

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTALEN_v2 (NG)

   Plasmid

   #32190

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

   Insert

   TALE Nuclease, NG for 0.5 repeat
   

   Expression

   Mammalian

   Available Since

   Dec. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTALEN_v2 (HD)

   Plasmid

   #32192

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

   Insert

   TALE Nuclease, HD for 0.5 repeat
   

   Expression

   Mammalian

   Available Since

   Dec. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. 56 pLIB_CBP

   Plasmid

   #160305

   Purpose

   N-term 3C cleavable CBP tag for insect cell expression

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   CBP

   Expression

   Insect

   Promoter

   polyhydrin

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. UBH14

   Plasmid

   #28158

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   E3 ligase UHRF2 (UHRF2 Human)
   

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   SRA Domain (residues 419-648)

   Available Since

   Feb. 28, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pNIC-MBP-TEV-GG

   Plasmid

   #215794

   Purpose

   Cloning of target gene with N terminal MBP tag and TEV cleavage

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET MBP mRuby2 LIC cloning vector (MBP-mRuby2)

    Plasmid

    #86933

    Purpose

    pET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mRuby2

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), MBP, TEV cleavage site, a…

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. 9 pCOLI_A_C_GST

    Plasmid

    #160245

    Purpose

    C-term GST uncleavable Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    GST

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. A-BRDA4-3

    Plasmid

    #166513

    Purpose

    scFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 333-460 of 1362, Bromodomain 2

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 52 pLIB_3C_C_STREP

    Plasmid

    #160300

    Purpose

    C-term 3C cleavable 2xStrepII tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    2xStrepII

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Nov. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Z-AIMP1-1

    Plasmid

    #166560

    Purpose

    scFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. NUDT4 (NUDT4A-c001)

    Plasmid

    #98241

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    NUDT4A (NUDT4 Human)
    

    Tags

    His-6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET24a MDH_STRAW

    Plasmid

    #204240

    Purpose

    Bacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    MDH_STRAW (mdh Streptomyces avermitilis)
    

    Tags

    6X His

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTALEN_v2 (NI)

    Plasmid

    #32189

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

    Insert

    TALE Nuclease, NI for 0.5 repeat
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNIC-HIS6-SUMO1-GG

    Plasmid

    #215799

    Purpose

    Cloning of target gene with N terminal His6 SUMO2 tag and SENP1 cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. PRDM11

    Plasmid

    #32858

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PRDM11 (PRDM11 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    amino acids 79-314

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pResQ shFEN3 3XF-FEN1 wt

    Plasmid

    #17752

    Depositor

    Sheila Stewart

    Insert

    Flap Endonuclease I (FEN1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Triple Flag

    Expression

    Mammalian

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only