Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 16,337 results for: LEA

Clear keywords
Showing: 8761 - 8780 of 16337 results

  1. HA-CPI-17-T38A-NLS

    Plasmid
    #100780
    Purpose
    Mammalian expression of CPI-17 neutral mutant nuclear
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38A-NLS (PPP1R14A Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    T38A
    Promoter
    CMV
    Available Since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100780-plasmid-map-sequence-id-193203

    Enlarge

  2. HA-CPI-17-T38E-NLS

    Plasmid
    #100778
    Purpose
    Mammalian expression of CPI-17 phospho-mimetic nuclear
    Depositor
    Joseph Gordon
    Insert
    CPI-17-T38E-NLS (PPP1R14A Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    T38E
    Promoter
    CMV
    Available Since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100778-plasmid-map-sequence-id-193198

    Enlarge

  3. pOpen-RNAse H

    Plasmid
    #165575
    Purpose
    Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    RNAse H
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165575-plasmid-map-sequence-id-340983

    Enlarge

  4. C33m

    Plasmid
    #120968
    Purpose
    MoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    AiiA degradase (Bennett, no ssra tag)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120968-plasmid-map-sequence-id-238044

    Enlarge

  5. pExp-NusA

    Plasmid
    #112571
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112571-plasmid-map-sequence-id-220666

    Enlarge

  6. pET28-MHL

    Plasmid
    #26096
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO (lactose/IPTG inducible)
    Available Since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    26096-plasmid-map-sequence-id-326568

    Enlarge

  7. pExp-Sumo

    Plasmid
    #112574
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112574-plasmid-map-sequence-id-217243

    Enlarge

  9. pCAGG-NFIB2

    Plasmid
    #112700
    Purpose
    Expression of NFIB2 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I B2 (Nfib Mouse)
    Tags
    GFP and HA
    Expression
    Mammalian
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112700-plasmid-map-sequence-id-212390

    Enlarge

  10. pCAGG-NFIC2

    Plasmid
    #112701
    Purpose
    Expression of NFIC2 protein and GFP
    Depositor
    Richard Gronostajski
    Insert
    Nuclear Factor I C2 (Nfic Mouse)
    Tags
    GFP and HA
    Expression
    Mammalian
    Available Since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    112701-plasmid-map-sequence-id-212389

    Enlarge

  11. pOpen-SfiIR (cDNA)

    Plasmid
    #165528
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    SfiI (used to create cDNA libraries)
    Use
    Synthetic Biology
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165528-plasmid-map-sequence-id-339067

    Enlarge

  12. pExp-NHis

    Plasmid
    #112558
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112558-plasmid-map-sequence-id-217235

    Enlarge

  13. pNIC28-Bsa4-TF

    Plasmid
    #61689
    Purpose
    E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences inserted
    Depositor
    Brian Smith
    Type
    Empty backbone
    Tags
    HIS and Trigger Factor
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pFBOH-MHL

    Plasmid
    #62304
    Purpose
    It is a donor vector for generation of recombinant baculovirus by site-specific transposition in an E. coli host.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    6xHis and TEV cleavage site
    Promoter
    polyhedrin
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62304-plasmid-map-sequence-id-102204

    Enlarge

  16. C79m

    Plasmid
    #120991
    Purpose
    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ccdA antitoxin
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120991-plasmid-map-sequence-id-236074

    Enlarge

  17. NET29b.Mm pEGFP-N2 (1327)

    Plasmid
    #62046
    Purpose
    mammalian expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET29b (Tmem120b Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62046-plasmid-map-sequence-id-101295

    Enlarge

  18. NET29A.Mm pEGFP-N2 (1326)

    Plasmid
    #62045
    Purpose
    mammalian expression of nuclear envelope transmembrane protein
    Depositor
    Eric Schirmer
    Insert
    NET29A (Tmem120a Mouse)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    62045-plasmid-map-sequence-id-101294

    Enlarge

  19. pETcon(I-LtrI)

    Plasmid
    #41523
    Depositor
    Andrew Scharenberg
    Insert
    I-LtrI
    Tags
    3x(G4S) Linker, G3S Linker, HA Tag, and Myc Tag
    Expression
    Yeast
    Promoter
    GAL1
    Available Since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    41523-plasmid-map-sequence-id-59600

    Enlarge

  20. C27m

    Plasmid
    #120965
    Purpose
    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    tetR (no deg tags)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120965-plasmid-map-sequence-id-236387

    Enlarge

Showing: 8761 - 8780 of 16337 results