We narrowed to 7,402 results for: Lif;

1. pX330_ACTB-i5 sgRNA / hSpCas9

   Plasmid

   #172829

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330_ACTB-i3 sgRNA / hSpCas9

   Plasmid

   #172827

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_ACTB-i2 sgRNA / hSpCas9

   Plasmid

   #172826

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUASTLOTattB_vhhGFP4::T48-Baz::mCherry

   Plasmid

   #163927

   Purpose

   Expression of GrabFP-A-extracellular under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 (extracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUASTLOTattB_mCherry::T48-Baz::vhhGFP4

   Plasmid

   #163928

   Purpose

   Expression of GrabFP-A-intracellular under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

   Plasmid

   #124066

   Purpose

   E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   cytATL-R232Q-sfGFP
   

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   R232Q

   Promoter

   trc

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR8/GW/TOPO-TBX20a

   Plasmid

   #98620

   Purpose

   Gateway entry TOPO TA cloning vector for TBX20a

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   Tbx20a (Tbx20 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MBP-ch-TOG-His6

   Plasmid

   #69108

   Purpose

   For bacterial expression of recombinant human ch-TOG tagged with MBP and His.

   Depositor

   Stephen Royle

   Article

   Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

   Insert

   Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. CLXSN 5myc-CDC6-WT

   Plasmid

   #109332

   Purpose

   express tagged Cdc6 WT

   Depositor

   Jean Cook

   Article

   Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

   Insert

   CDC6 (CDC6 Human)
   

   Use

   Retroviral

   Tags

   5x Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pInducer20-Blast-CDT1-HA

    Plasmid

    #109335

    Purpose

    virus packaged, doxycyline inducible expression of Cdt1

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Insert

    Cdt1 (CDT1 Human)
    

    Use

    Lentiviral; Inducible expression

    Tags

    HA, His, Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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