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Plasmid#63834PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-11
Plasmid#63836PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640a-1
Plasmid#42092DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 820-842 nt segment of ZEB1 mRNA (NM_…PromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640s-3
Plasmid#42091DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 4565-4587 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-302s-1
Plasmid#42087DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 4146-4168 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-215a-1
Plasmid#42085DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 1259-1281 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640s-2
Plasmid#42090DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 1956-1981 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640s-1
Plasmid#42089DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 1419-1441 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-302a-1
Plasmid#42088DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 4055-4078 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640a-2
Plasmid#42093DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 1635-1657 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-215a-2
Plasmid#42086DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 2444-2466 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCT310
Plasmid#111140PurposeBacterial expression of 6His-dCas9-3XFlagDepositorInsertdCas9
UseTags3X Flag and 6X HisExpressionBacterialMutationD10A - H840APromoterT7Available sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat-ADE2
Plasmid#232103PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pGCP123-GFP_g1
Plasmid#153518PurposesgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcodedDepositorInsertPnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGCP123-EbpA_g1
Plasmid#153517PurposesgRNA for ebpA gene (NT) under nisin-inducible nisA promoter, barcodedDepositorInsertpnisA-sgRNA(EbpA_g1)-dCas9 scaffold
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1.mutant
Plasmid#128746PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR.DepositorInsertCyrano conserved region (CR1) with mutant miR-7 site (OIP5-AS1 Human)
UseTagsExpressionMammalianMutation5 point mutations disrupting the miR-7 sitePromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
TL1 alphaL_DdCBE 1-2-3 (right, DddA11-N)
Plasmid#221997PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-1/2/3 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationDddA: S1330I, A1341V, N1342S, E1370K, T1380IPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1.miR16
Plasmid#128747PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.DepositorInsertCyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-NS1-YN
Plasmid#74723PurposeExpress C-terminal YN fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsHA and YFP N-terminal half domainExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only