Addgene

We narrowed to 6,461 results for: poly

Showing: 861 - 880 of 6461 results

Results per page:

Sort results by:

pTK312

Plasmid

#59569

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP XbaI attB1 Kozak EGFP ochre attB2 AscI

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCSG28-SH3-588

Plasmid

#185920

Purpose

Expresses SH3-588, a designed poly-proline peptide recruiting catechol O-methyltransferase

Depositor

Brian Kuhlman

Article

Park et al Nat Chem Biol. 2023 Apr;19(4):460-467. d

Insert

SH3-588

Use

Tags

6x HIS tag

Expression

Bacterial

Mutation

Designed enzyme with novel interface between huma…

Promoter

T7

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-dSa-VPR mini.-syn pA

Plasmid

#99689

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Expression

Mutation

dead Cas9

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pG23A

Plasmid

#102884

Purpose

Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Insert

Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)

Use

Tags

AID(71-114) and mCherry

Expression

Yeast

Mutation

Promoter

Native promoter

Available since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro-PARG shRNA

Plasmid

#23260

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan 12. ():.

Insert

poly(ADP-ribose) glycohydrolase shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
pCS2_Firefly_degron-60A

Plasmid

#200396

Purpose

A plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

Depositor

Xiao Wang

Article

Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

Insert

Firefly luciferase - degron

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pK RagA(Q66L)-RagC

Plasmid

#99700

Purpose

Bacterial expression of RagA(Q66L)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

L91P

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEP223-AAV-EFS-GFP-pA

Plasmid

#62913

Purpose

AAV vector backbone designed to express GFP from a EFS promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Tags

Expression

Mutation

Promoter

short form of Elongation Factor1α (EFS)

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pK RagA-RagC

Plasmid

#99649

Purpose

Bacterial expression of wildtype Rag GTPase dimer

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

10x His tag, Poly arginine linker, and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT100-neo

Plasmid

#123137

Purpose

Golden Gate donor vector for the assembly of EGFP-based mammalian recombinase reporters.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Neomycin, poly-A terminator

Use

Synthetic Biology; Golden gate donor vector

Tags

Expression

Mutation

Promoter

none

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-dSa-VPR mini.-1X snRP1

Plasmid

#99687

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Expression

Mutation

dead Cas9

Promoter

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pK RagA-RagC(Q120L)

Plasmid

#99701

Purpose

Bacterial expression of RagA-RagC(Q120L)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

Q120L

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Dendra2-NS2B

Plasmid

#204218

Purpose

Expresses Dendra2-NS2B in Mammalian / Mouse Cell Lines

Depositor

Partha Mondal

Article

Aravinth et al Sci Rep. 2025 Mar 26;15(1):10358. doi: 1

Insert

Dengue (Dengue Type 2) NS2B (POLY Dengue Virus Serotype , Strain NGC)

Use

Tags

Dendra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCD-HCVP

Plasmid

#226227

Purpose

Express HCVP

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

HCVP (POLY Hepatitis C virus)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEP224-AAV-Basic-0.4αCaMKII-GFP-pA

Plasmid

#62909

Purpose

AAV basic vector backbone designed to express GFP from a 0.4CaMKIIa promoter. It also contains a poly-Adenylation Signal (pA).

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Tags

Expression

Mutation

Promoter

0.4αCaMKII

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJEP221-AAV-RSV-GFP-pA

Plasmid

#62919

Purpose

AAV vector backbone designed to express GFP from a RSV promoter. It also contains a poly-Adenylation Signal (pA)

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

AAV

Tags

Expression

Mutation

Promoter

Rous Sarcoma Virus(RSV)

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3TS-Hypa-nCas9n-A69

Plasmid

#125645

Purpose

micro-injection of poly-adenylated Hypa-nCas9n RNA

Depositor

Jason Berman

Article

Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

Insert

Hypa-nCas9n

Use

Rna in vitro transcription vector

Tags

Expression

Mutation

N692A, M694A, Q695A, H698A from wild-type Cas9

Promoter

T3

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pK RagA(R37P)-RagC

Plasmid

#99706

Purpose

Bacterial expression of RagA(R37P)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

R37P

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pK RagA-RagC(L91P)

Plasmid

#99707

Purpose

Bacterial expression of RagA-RagC(L91P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

L91P

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pK RagA-RagC(K84T)

Plasmid

#99708

Purpose

Bacterial expression of RagA-RagC(K84T)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

K84T

Promoter

T7

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge