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pFa6-TID-FRB-3HA-KanMX6

Plasmid

#228020

Purpose

For TurboID-FRB integration; yeast BioID with KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-TID-FRB-3HA-HIS3MX6

Plasmid

#228021

Purpose

For TurboID-FRB integration; yeast BioID with HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-BirA-FRB-HA3

Plasmid

#227984

Purpose

Expresses BirA-FRB fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA-FRB

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-BirA-FKBP-HA3

Plasmid

#227986

Purpose

Expresses BirA-FKBP fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA-FKBP

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-BioID2-FRB-HA3

Plasmid

#227988

Purpose

Expresses BioID2-FRB fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BioID2-FRB

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-ADH1p-BioID2-FKBP-HA3

Plasmid

#227990

Purpose

Expresses BioID2-FKBP fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BioID2-FKBP

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-ADH1p-BASU-FRB-HA3

Plasmid

#227992

Purpose

Expresses BASU-FRB fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU-FRB

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-ADH1p- BASU-FKBP-HA3

Plasmid

#227994

Purpose

Expresses BASU-FKBP fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU-FKBP

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-ADH1p-mTID-FRB-HA3

Plasmid

#228000

Purpose

Expresses miniTurboID-FRB fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-ADH1p-mTID-FKBP-HA3

Plasmid

#228002

Purpose

Expresses miniTurboID-FKBP fusion from ADH1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

miniTurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-HIS3p-TID-FRB-HA3

Plasmid

#228004

Purpose

Expresses TurboID-FRB fusion from HIS3 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

HIS3

Available Since

Nov. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416-CHA1p-TID-FKBP-HA3

Plasmid

#228014

Purpose

Expresses TurboID-FKBP fusion from CHA1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

CHA1

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-MET25p-TID-FRB-HA3

Plasmid

#228016

Purpose

Expresses TurboID-FRB fusion from MET25 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-MET25p-TID-FKBP-HA3

Plasmid

#228018

Purpose

Expresses TurboID-FKBP fusion from MET25 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

MET25

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-BASU-HA3-KanMX6

Plasmid

#227978

Purpose

To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, KanMX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU

Tags

HA tag

Expression

Yeast

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pML107-PMR1

Plasmid

#226264

Purpose

Plasmid expressing Cas9 and gRNA ACATGACCGTATCTAAACTT which targets the PMR1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

PMR1 gRNA (PMR1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pML107-LUG1

Plasmid

#226265

Purpose

Plasmid expressing Cas9 and gRNA TCTTCAAGTTACTCCAAGAG which targets the LUG1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LUG1 gRNA (YLR352W Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pML104-LEU2-2

Plasmid

#226263

Purpose

Plasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

LEU2 gRNA (LEU2 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM032_P4_tSynth30

Plasmid

#216457

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth30

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM031_P4_tSynth29

Plasmid

#216456

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth29

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM030_P4_tSynth27

Plasmid

#216455

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth27

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM029_P4_tSynth25

Plasmid

#216454

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth25

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM028_P4_tSynth3

Plasmid

#216453

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOM027_P4_tSynthGuo

Plasmid

#216452

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Article

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator guo

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK26 pHomL-TEF2p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179010

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK25 pHomL-TDH3p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179009

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK96 pHomL-ConLS-GFPdropout-ConR1-HomR (AmpR)

Plasmid

#179080

Purpose

Parent vector for for creating new markerless yeast integration cassettes with different promoters/genes/tags/terminators

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK42 pHomL-RPL18Bp-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)

Plasmid

#179026

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK41 pHomL-TEF2p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)

Plasmid

#179025

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK40 pHomL-TDH3p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)

Plasmid

#179024

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genes

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge