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pKLV2-U6gRNA5(Znrf1-g1)-PGKpuroBFP-W

Plasmid

#105020

Purpose

Lentiviral gRNA plasmid targeting mouse Znrf1 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Znrf1 (Znrf1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Nod-BFP2-TSERex

Plasmid

#124785

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Nod-BFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-V111FL114FI766F-HA-TagBFP

Plasmid

#120911

Purpose

Expresses BFP tagged Ptch1-B (V111FL114FI766F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Use

Tags

HA and TagBFP

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, Y…

Promoter

CMV

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSB-Bleo Gal4 tBFP

Plasmid

#139190

Purpose

Gal4-inducible tag blue fluorescent protein (tBFP) in pSB-Bleo Gal4 Sleeping Beauty backbone.

Depositor

Neal Devaraj

Insert

tBFP

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

Gal4 CMV and STAT3

Available since

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-EBFP2-NLS (pRW1447)

Plasmid

#216219

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCCL-PGK-SPdCas9-BFP-DNMT1

Plasmid

#66818

Purpose

dCas9 fused to BFP and the human DNMT1 catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dCas9-BFP-DNMT1, catalytic domain (DNMT1 )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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EK0396 BFPmut-stop-t70587 (pEAQ)

Plasmid

#191171

Purpose

EK0208 in plant-expressable format; Trigger 1 in 3' UTR of an inactive BFP

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

t70587 (plant)

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p-EF1a-CreERT2-3Xflag-T2A-eBFP2

Plasmid

#170186

Purpose

This Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracing

Depositor

Bradley Bernstein

Article

Kim et al Cell Rep. 2020 Oct 6;33(1):108222. doi:

Insert

CreERT2-T2A-eBFP2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1 alpha

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP

Plasmid

#60903

Purpose

Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 peptide v4 and BFP. This plasmid is part of the SunTag system for gene activation

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

Cas9 dead

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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CAG-BFP-sesRNA-GFP-WPRE-pA

Plasmid

#192063

Purpose

Expression of BFP, sesRNA in mammalian cells, with GFP as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for tdTom

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-dCas9-BFP-KRAB

Plasmid

#46911

Purpose

Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-BFP-KRAB fusion

Use

CRISPR and Lentiviral

Tags

2xNLS, BFP, HA, and KRAB domain

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-puro-cOVA-IRES-BFP

Plasmid

#135074

Purpose

Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance. cOVA is coupled with BFP fluorescent reporter using IRES.

Depositor

Maria Castro

Insert

cOVA-IRES-BFP (OVAL Chicken)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-UCOE-dCas9-BFP-VP64

Plasmid

#196717

Purpose

CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

Depositor

Bernhard Schmierer

Insert

dCas9-TagBFP-VP64

Use

CRISPR and Lentiviral

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mutation

D10A, N863A

Promoter

EF1a

Available since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a-P2A-tagBFP

Plasmid

#84569

Purpose

Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Human, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pREP-8xARE-GFP-SV40-BFP

Plasmid

#134910

Purpose

NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Inserts

8xARE- minimal promoter - GFP
TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

8xARE - minimal promoter and SV40

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

Plasmid

#190181

Purpose

Fluorescent reporter vector to visualize all of cell cycle phases.

Depositor

Toshiro Sato

Article

Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

Inserts

iRFP
hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
TagBFP2
hGeminin(1/110)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD-P2A-BFP

Plasmid

#108245

Purpose

Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A

Promoter

Ubc

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNASAM(gTetO)-TREGFP-PGKpuroBFP-W

Plasmid

#112927

Purpose

CRISPR-a reporter assay lentiviral vector (gTetO)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6-gRNASAM(gTetO), TREGFP, PGKpuroGFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAF0689 Ef1a-Pa01_attB-BFP acceptor

Plasmid

#193472

Purpose

Acceptor plasmid for 3 plasmid recombination assay

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01_attB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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