We narrowed to 16,131 results for: form

1. pFRT-DHX36iso1E335A

   Plasmid

   #159587

   Purpose

   Express N-ter FLAG HA tagged DHX36 isoform 1 with E335A mutation in mammalian cells

   Depositor

   Markus Hafner

   Article

   Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

   Insert

   DHX36 (DHX36 Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   E335A

   Promoter

   CMV

   Available Since

   May 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. retro-puro vector

   Plasmid

   #58250

   Purpose

   Retroviral expression vector encoding an empty IRES-Puromycin cassette

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-pHSE::Cre-mRuby3

   Plasmid

   #164136

   Purpose

   Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   HSE promoter-Cre-T2A-mRuby3
   

   Use

   CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

   Expression

   Mammalian

   Promoter

   HSE synthetic promoter

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_ER-RE_MLPmin_BC1063-luc2

   Plasmid

   #227109

   Purpose

   Estrogen receptor response element for luciferase and barcode assays; barcode BC1063

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered ER response element linked to MLP minimal promoter driving barcode 1063 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCIG AKT3 E17K

   Plasmid

   #73050

   Purpose

   Human AKT3 E17K

   Depositor

   Joseph Gleeson

   Article

   Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

   Insert

   AKT3 E17K (AKT3 Human)
   

   Expression

   Mammalian

   Mutation

   E17K

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLAP-BUBR1

   Plasmid

   #114052

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   BUBR1 (BUB1B Human)
   

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance)

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry

   Plasmid

   #35502

   Purpose

   AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   hChR2(C128S/D156A)-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   C128S and D156A

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. PiggyBac-FLAG-mProser1-IRES-Blast

   Plasmid

   #226172

   Purpose

   Expresses full length mouse PROSER1 with 2X N-terminal FLAG tags

   Depositor

   Kasper Rasmussen

   Article

   Fleming et al Genes Dev. 2024 Nov 19. doi: 10.1101/gad

   Insert

   Proline Serine Rich protein 1 (Proser1 Mouse)
   

   Tags

   2X FLAG

   Expression

   Mammalian

   Available Since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Zeo_BACE1

   Plasmid

   #227105

   Purpose

   BACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assays

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   BACE1 (BACE1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-TRE-FH-TET3 short

    Plasmid

    #166916

    Purpose

    Lentivirus for inducible overexpression of human TET3 short isoform lacking CXXC domain (NM_001366022.1)

    Depositor

    Roland Friedel

    Article

    Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

    Insert

    TET3 short isoform
    

    Use

    Lentiviral

    Tags

    HA Tag

    Expression

    Mammalian

    Mutation

    Changed nucleotide 18 (G) to (A) to delete FseI r…

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-TetOn-3XFLAG-renilla-beta-globin-control-AAVS1

    Plasmid

    #184396

    Purpose

    Donor plasmid for stable integration of Renilla luciferase control reporter at AAVS1

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase beta-globin
    

    Use

    CRISPR; Donor plasmid for hdr

    Tags

    3X FLAG

    Expression

    Mammalian

    Promoter

    Tet-On 3G

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-hSyn-Galpha-s ONE-GO

    Plasmid

    #214371

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    Galpha-s ONE-GO (GNAS Human)
    

    Use

    Lentiviral

    Promoter

    hSyn

    Available Since

    Feb. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pαH-S-GSAS

    Plasmid

    #164565

    Purpose

    SARS-CoV-2 Spike protein (S-GSAS variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-GSAS variant)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. tetO-Bcl2-IRES-tdtomato(TET005)

    Plasmid

    #117857

    Purpose

    This plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

    Depositor

    C. Ron Yu

    Article

    Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

    Insert

    Bcl2 (Bcl2 Mouse)
    

    Use

    Tta/teto

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST17-cDAXX

    Plasmid

    #169729

    Purpose

    Bacterial expression of N-terminally 6His tagged cDAXX

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    DAXX (DAXX Human)
    

    Tags

    6 His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. hIL23p19 pEF6-V5 (MJS_004)

    Plasmid

    #72492

    Purpose

    Expression of human IL23p19 in mammalian cells (V5 tag)

    Depositor

    Matthew Sweet

    Article

    Ramnath et al Immunol Cell Biol. 2015 Oct;93(9):771-9.

    Insert

    IL23p19 (IL23A Human)
    

    Tags

    His/V5

    Expression

    Mammalian

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG mFoxg1-GFP wildtype

    Plasmid

    #73048

    Purpose

    C-terminal fusion of gfp Mouse wildtype Foxg1

    Depositor

    Joseph Gleeson

    Article

    Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

    Insert

    mFoxg1 (Foxg1 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-Rab8a-DN (T22N)

    Plasmid

    #101049

    Purpose

    Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    Rab8a (RAB8A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T22N

    Promoter

    CMV

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFPV25-cR-2iG

    Plasmid

    #204618

    Purpose

    Fluorescent reporter for SPI2 induction in Salmonella

    Depositor

    Jacques Neefjes

    Article

    Stévenin et al Cell Rep. 2024 Nov 1;43(11):114931. doi:

    Inserts

    rpsM promoter
    sseA promoter
    

    Tags

    GFP and dsRed

    Expression

    Bacterial

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-CMV 3xFLAG-LC3A-GFP

    Plasmid

    #123106

    Purpose

    Expresses 3xFLAG-LC3A-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3A

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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