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pT7-EGFP-C1-HCV-RLuc-6xbsMS2-polyA-Malat_AC

Plasmid

#148448

Purpose

Mammalian Expression of 6xbsMS2-polyA-Malat

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

6xbsMS2-polyA-Malat

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF1_AC

Plasmid

#148449

Purpose

Mammalian Expression of HseIF1

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HseIF1 (ELF1 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-E236Q-shRNAres_AC

Plasmid

#148450

Purpose

Mammalian Expression of HSRck-E236Q-shRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HSRck-E236Q-shRNAres (DDX6 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-K135A-shRNAres_AC

Plasmid

#148451

Purpose

Mammalian Expression of HsRck-K135A-shRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-K135A-shRNAres (DDX6 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-QHTRRKKtoAAAAAAA-shRNAres_AC

Plasmid

#148452

Purpose

Mammalian Expression of HsRck-QHTRRKKtoAAAAAAA-shRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-QHTRRKKtoAAAAAAA-shRNAres (DDX6 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-QHTRtoAAAA-shRNAres_AC

Plasmid

#148453

Purpose

Mammalian Expression of HsRck-QHTRtoAAAA-shRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-QHTRtoAAAA-shRNAres (DDX6 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-RFQNRtoEDAAA-shRNAres_AC

Plasmid

#148454

Purpose

Mammalian Expression of HsRck-RFQNRtoEDAAA-shRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-RFQNRtoEDAAA-shRNAres (DDX6 Human)

Expression

Mammalian

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-mCCK2R-stop

Plasmid

#179321

Purpose

Express mouse cholecystokinin 2 receptor (untagged) in mammalian cells

Depositor

Christian Gruber

Publication

Taghizadeh et al Sci Rep. 2022 Jun 2;12(1):9215. doi: 10.

Insert

mouse cholecystokinin2 receptor

Expression

Mammalian

Mutation

stop codon after ORF

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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IL2-mCherry-AH in pmCherryN1

Plasmid

#187284

Purpose

Express IL2-AH-mCherry

Depositor

Alpha Yap

Publication

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)

Tags

mCherry

Expression

Mammalian

Mutation

AH

Promoter

CMV

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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ATX3-JD

Plasmid

#184247

Purpose

Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)

Depositor

Sandra Macedo Ribeiro

Publication

Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

Insert

Ataxin-3 Josephin domain (ATXN3 Human)

Tags

6x His-Tag and TEV cleavage site

Expression

Bacterial

Mutation

Premature stop codon V183X

Promoter

T7 promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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rtTA-sg-tdTomato166/892

Plasmid

#188488

Purpose

gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.

Depositor

Indeever Madireddy, Johan Sosa

Publication

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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rtTA-sg-tdTomato90/816

Plasmid

#188487

Purpose

gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.

Depositor

Indeever Madireddy, Johan Sosa

Publication

Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

Insert

tdTomato sgRNA

Use

CRISPR

Expression

Mammalian

Mutation

None

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-GSSGmut-dsRNAres_Q

Plasmid

#147338

Purpose

Insect Expression of DmDCP1-GSSGmut-dsRNAres

Depositor

Elisa Izaurralde

Publication

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmDCP1-GSSGmut-dsRNAres (Dcp1 Fly)

Expression

Insect

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK26 pHomL-TEF2p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179010

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK25 pHomL-TDH3p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)

Plasmid

#179009

Purpose

Parent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-sfGFP

Plasmid

#180756

Purpose

pET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Publication

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET3a-FruK

Plasmid

#186256

Purpose

Overexpression of FruK for purification

Depositor

Liskin Swint-Kruse, Emile Van Schaftingen

Publication

Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

Insert

1-phosphofructokinase (fruK E. coli)

Expression

Bacterial

Promoter

T7

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP.N3-myc-SLC35A2

Plasmid

#186285

Purpose

transient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Publication

Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

Insert

SLC35A2 (SLC35A2 Human)

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCJH026_proD_Cas12c-multiplexing_CAM

Plasmid

#183073

Purpose

Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression

Depositor

Jennifer Doudna

Publication

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript

Use

CRISPR

Expression

Bacterial

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-10-mCherry-R11b

Plasmid

#179891

Purpose

Donor with homologous arms for Rab11b to knock in mCherry

Depositor

Patrick Caswell

Publication

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11B, member RAS oncogene family (RAB11B Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mCherry

Expression

Bacterial and Mammalian

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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