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pLEW100v5-BSD-FLAG-La-Cas9

Plasmid

#245102

Purpose

Inserts tetracycline-inducible codon optimized, nuclceus targeted FLAG-Cas9 into T. brucei cells at the rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

3xFLAG-La-Cas9

Use

CRISPR

Tags

3xFLAG-La

Mutation

codon optimized for T. brucei

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.100bp_offset

Plasmid

#245115

Purpose

Inserts 100 bp of VSG-228 coding sequence in T. brucei rDNA spacer overlapping AnTat1.1 position 694, centered around common VSG-228 insertion

Depositor

Monica Mugnier

Article

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Insert

VSG-228:100_offset

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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NSE-huCof K22Q-mRFP

Plasmid

#252618

Purpose

Neuronal specific expression of a mRFP tagged human cofilin K22Q (rod reporter)

Depositor

James Bamburg

Article

Mi et al PLoS One. 2013 Dec 31;8(12):e83609. doi:

Insert

cofilin 1 (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

Neuronal Specific Enolase (NSE)

Available Since

March 6, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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huCofilin WT SSR

Plasmid

#252616

Purpose

Expresses human wild-type cofilin (shRNA-resistant)

Depositor

James Bamburg

Article

Wiggan et al Dev Cell. 2012 Mar 13;22(3):530-43. doi:

Insert

cofilin 1 (CFL1 Human)

Expression

Mammalian

Mutation

silent mutations at nt 67 to 75 in which TCTTCAAC…

Promoter

CMV

Available Since

March 5, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti X1 Puro 8xGLI_minPro_Luc2p_Ren

Plasmid

#249282

Purpose

Dual-luciferase GLI reporter viral plasmid. 8xGLI binding site followed by minPro-luc2P expression, in addition to constitutive SV-40-driven Renilla luciferase expression.

Depositor

Ramiro Iglesias-Bartolome

Article

Krantz et al Cancer Res. 2025 Dec 29. doi: 10.1158/00

Insert

Luciferase

Use

Lentiviral

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pX330-TAF1-A

Plasmid

#247346

Purpose

Expresses SpCas9 and a sgRNA targeting the human TAF1-A loci for knock-in.

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

TAF1 (TAF1 Human)

Use

CRISPR

Promoter

U6

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2865

Plasmid

#232514

Purpose

Expression plasmid of split-LetA with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV and StrepTagII

Expression

Bacterial

Mutation

[6xHis2xQH-TEV-LetA1(1-223)]-[LetA2(224-427)-Stre…

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2870

Plasmid

#232515

Purpose

Genetic complementation plasmid of Cys-light LetA(L94C,I383C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

[LetA1(1-223, L94C,C124S)]-[LetA2(224-427,I383C,C…

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2871

Plasmid

#232516

Purpose

Genetic complementation plasmid of Cys-light LetA(Q180C,R380C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

[LetA1(1-223,Q180C,C124S)]-[LetA2(224-427,R380C,C…

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2874

Plasmid

#232517

Purpose

Expression plasmid of Cys-light LetA(L94C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

[6xHis2xQH-TEV-LetA1(1-223,L94C,C124S)]-[LetA2(22…

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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