We narrowed to 15,736 results for: LEA

1. pET28a MDHC_YEAST

   Plasmid

   #204232

   Purpose

   Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDHC_YEAST (MDH2 Budding Yeast)
   

   Tags

   6X His

   Expression

   Bacterial

   Promoter

   Lac

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PGK-AAVS1ZFNR

   Plasmid

   #60915

   Purpose

   encodes zinc finger nuclease specific to AAVS1 locus (right)

   Depositor

   Paul Gadue

   Article

   Sim et al Methods Mol Biol. 2015 Jan 29.

   Insert

   AAVS1-ZFNR
   

   Promoter

   PGK

   Available Since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.CAG.(mem).iGlucoSnFR2

   Plasmid

   #244077

   Purpose

   Plasma membrane targeted expression of green glucose sensor

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   iGlucoSnFR2
   

   Use

   AAV

   Tags

   IgG kappa leader

   Available Since

   Sept. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

   Plasmid

   #51083

   Purpose

   Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Has Service

   AAV Retrograde

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Promoter

   hEF1a

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. PGK-AAVS1ZFNL

   Plasmid

   #60916

   Purpose

   encodes zinc finger nuclease specific to AAVS1 locus (left)

   Depositor

   Paul Gadue

   Article

   Sim et al Methods Mol Biol. 2015 Jan 29.

   Insert

   AAVS1-ZFNL
   

   Promoter

   PGK

   Available Since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROPseq-multi-EBFP2-NLS (pRW1447)

   Plasmid

   #216219

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 20 pCOLI_A_N-His_C_STREP

   Plasmid

   #160162

   Purpose

   N-term 6xHis 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   2xStrepII and 6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSSA-1-3

   Plasmid

   #35091

   Depositor

   David Segal

   Article

   Bhakta et al Methods Mol Biol. 2010;649:3-30.

   Insert

   Split luciferase for GZF1/3 SSA assay
   

   Expression

   Mammalian

   Mutation

   Single strand annealing (SSA) recombinaion report…

   Promoter

   SV40

   Available Since

   March 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

   Plasmid

   #51085

   Purpose

   Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

   Depositor

   Jonathan Ting

   Has Service

   AAV Retrograde and AAV1

   Insert

   GCaMP6f
   

   Use

   AAV

   Tags

   P2A-nls-dTomato

   Expression

   Mammalian

   Promoter

   human Synapsin1

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

    Plasmid

    #51084

    Purpose

    Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde and AAV1

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Promoter

    human Synapsin1

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. EBFP2-LaminB1-10

    Plasmid

    #55244

    Purpose

    Localization: Nuclear Envelope, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    EBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. mPlum-LaminB1-10

    Plasmid

    #55981

    Purpose

    Localization: Nuclear Envelope, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    mPlum

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pRSETA_ionRD

    Plasmid

    #172924

    Purpose

    Ionic strength FRET sensor with mCerulean3 and mCitrine for E. coli expression.

    Depositor

    Arnold Boersma

    Article

    Liu et al ACS Chem Biol. 2017 Oct 20;12(10):2510-2

    Insert

    IonicStrengthSensorRD
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

    Plasmid

    #51082

    Purpose

    Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV1

    Insert

    GCaMP6s
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Promoter

    hEF1a

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. tdTomato-LaminB1-10

    Plasmid

    #58107

    Purpose

    Localization: Nuclear Envelope, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. LeGO-iCer

    Plasmid

    #27363

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    U6 promoter, SFFV promoter, IRES-Cerulean
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. mApple-LaminB1-10

    Plasmid

    #54917

    Purpose

    Localization: Nuclear Envelope, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    LaminB1 (LMNB1 Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pAd nGFP

    Plasmid

    #19413

    Depositor

    Douglas Melton

    Article

    Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

    Insert

    nuclear GFP
    

    Use

    Adenoviral; Entry vector

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

19. LeGO-iCer2

    Plasmid

    #27346

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, IRES-Cerulean
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTrcHisA-PPARalpha(aa101-468)

    Plasmid

    #13574

    Depositor

    Martin Scanlon

    Insert

    human peroxisome proliferator activated receptor alpha A/B domain deletion construct (aa101-468) (PPARA Human)
    

    Tags

    x6His N-terminal, enterokinase cleavage site

    Expression

    Bacterial

    Mutation

    deleted residues 1-100.

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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