We narrowed to 15,910 results for: grna

Showing: 8941 - 8960 of 15910 results

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pTBL511 CC sgRNA

Plasmid

#126431

Purpose

To target a protospacer with CC at the cut site.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with CC at cut site

Expression

Mammalian

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL508 TA sgRNA

Plasmid

#126438

Purpose

To target a protospacer with TA at the cut site.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with TA at cut site

Expression

Mammalian

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL250 TC sgRNA

Plasmid

#126439

Purpose

To target a protospacer with TC at the cut site.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with TC at cut site

Expression

Mammalian

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL514 TG sgRNA

Plasmid

#126440

Purpose

To target a protospacer with TG at the cut site.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with TG at cut site

Expression

Mammalian

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pTBL513 TT sgRNA

Plasmid

#126441

Purpose

To target a protospacer with TT at the cut site.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

Insert

spacer against human genome with TT at cut site

Expression

Mammalian

Promoter

human U6

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pX330-MGAT1-KO

Plasmid

#80009

Purpose

gRNA to knock out expression of MGAT1 gene. The product of this gene is essential for synthesis of complex and hybrid N-Glycans.

Depositor

Sriram Neelamegham

Publication

Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

Insert

MGAT1 (MGAT1 Human)

Use

CRISPR

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA

Plasmid

#218710

Purpose

AAV vector plasmid expressing Cas9 from Staphylococcus aureus (SaCas9) under the human synapsin (SYN) promoter and its sgRNA under the U6 promoter

Depositor

Bart Nieuwenhuis

Inserts

hSaCas9
Chimeric guide for SaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAT2981-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank6_PC3 DM_AT_sg-227-233])

Plasmid

#251111

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Publication

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pAT2977-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank1_PC3 DM_AT_sg-213-219])

Plasmid

#251109

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Publication

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAT2978-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank2_PC3 DM_AT_sg-234-240])

Plasmid

#251110

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Publication

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pAT2982-(PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank7_PC3 DM_AT_sg-241-247])

Plasmid

#251112

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Publication

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)

Plasmid

#126778

Purpose

Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

HiFi SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R691A

Promoter

CBh

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMC-Dest-uni-gRNA5-BsaI

Plasmid

#241150

Purpose

Destination vector for mini-Golden subcloning platform; contains universal sgRNA at 5' end

Depositor

Junsu Kang

Publication

Rodriguez-Parks et al Biol Open. 2026 Feb 2:bio.062472. doi: 1

Type

Empty backbone

Use

Minicircle cloning vector

Mutation

TCGC linker

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4b Bxb1 attB38

Plasmid

#242879

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 site

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for Bxb1 attB site

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4a Bxb1 attB38

Plasmid

#242878

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 site

Depositor

Jesse Owens

Publication

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for Bxb1 attB site

Expression

Mammalian

Mutation

none

Promoter

U6

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR3-SV40-puro

Plasmid

#240537

Purpose

Lentiviral eBAR3 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Publication

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR3

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-U6-epegRNA eBAR2-SV40-puro

Plasmid

#240536

Purpose

Lentiviral eBAR2 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.

Depositor

Wensheng Wei

Publication

Niu et al Nat Biotechnol. 2025 Jun 24. doi: 10.103

Insert

epegRNA-eBAR2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Donor-attp-u6-blank-sgrna-backbone

Plasmid

#234076

Purpose

Blank donor for Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

Depositor

Gary Hon

Publication

Sivakumar et al Stem Cell Reports. 2025 Dec 9;20(12):102

Insert

sgRNA-Blank

Use

Lentiviral

Expression

Mammalian

Available since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti pTERT sgRNA2(MS2) mCherry

Plasmid

#171055

Purpose

Targeting of Vp64-dCas9 activation system to hTERT promoter

Depositor

Agnel Sfeir

Publication

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

pTERT sgRNA2(MS2) mCherry

Use

Lentiviral

Expression

Mammalian

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

Plasmid

#181790

Purpose

Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ

Expression

Bacterial

Mutation

nAniI = K227M, F80K, L232K

Promoter

Lac and J23119

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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