We narrowed to 9,127 results for: Pol

1. MZB211

   Plasmid

   #228494

   Purpose

   10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   pol
   

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Mutation

   A408S

   Promoter

   T7 Promoter

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOpen-T7gene5

   Plasmid

   #165534

   Purpose

   DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   T7 DNA Polymerase (unmodified)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. LHP1048 - pFB-RSP5

   Plasmid

   #32434

   Depositor

   Linda Hicke

   Insert

   RSP5 (RSP5 Budding Yeast)
   

   Expression

   Insect

   Promoter

   P-P10 and P-Pol

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

4. PJD1223

   Plasmid

   #160390

   Purpose

   Encodes L-A cDNA for curing L-A virus and Parent for pJD1223-NAT

   Depositor

   John McCusker

   Insert

   L-A Gag-Pol
   

   Expression

   Yeast

   Promoter

   TRP1

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV delta R8.2

   Plasmid

   #12263

   Purpose

   First-generation packaging plasmid. This plasmid contains HIV accessory genes. Please reach out to your biosafety officer for guidance.

   Depositor

   Didier Trono

   Insert

   HIV-1 GAG/POL, Tat and Rev
   

   Use

   Lentiviral; Packaging

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. Ec (gamma-only)-pET(11a)(ACH–)

   Plasmid

   #241265

   Purpose

   Over-express E. coli Pol III subunit gamma-only in E. coli

   Depositor

   Michael O'Donnell

   Insert

   dnaXZ (gamma-only)
   

   Expression

   Bacterial

   Mutation

   No expression of tau

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAS-mak10 gRNA

   Plasmid

   #160385

   Purpose

   Expresses guide RNA for targeting MAK10 in yeast

   Depositor

   John McCusker

   Insert

   mak10 gRNA
   

   Expression

   Yeast

   Promoter

   RNA pol III promoter (tRNA-Tyr)

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p23-puro-EGFP-RPA194

   Plasmid

   #179237

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   RPA194 (POLR1A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF-1alpha

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p23-mNeongreen-DDX21 WT

   Plasmid

   #179228

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   DDX21 (DDX21 Human)
   

   Tags

   mNeongreen

   Expression

   Mammalian

   Promoter

   EF-1alpha

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Seattle_IPDA_control_6_002

    Plasmid

    #167348

    Purpose

    ddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    pol_gag_tat
    

    Use

    Other

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Seattle_IPDA_control_29_006

    Plasmid

    #167352

    Purpose

    ddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    pol_env
    

    Use

    Other

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. p23-mNeongreen-DDX21 WT-mRuby3

    Plasmid

    #179225

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Tags

    mNeongreen and mRuby3

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p23-mRuby3-DDX21 WT

    Plasmid

    #179231

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Tags

    mRuby3

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. p23-mNeongreen-DDX21 DAT-mRuby3

    Plasmid

    #179226

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Tags

    mNeongreen and mRuby3

    Expression

    Mammalian

    Mutation

    G234D, K235A

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. p23-mNeongreen-DDX21 HGV-mRuby3

    Plasmid

    #179227

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Tags

    mNeongreen and mRuby3

    Expression

    Mammalian

    Mutation

    D338H, E339G

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PGEX-DDX21-D2+C

    Plasmid

    #179243

    Purpose

    protein purification in E. coli

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21-D2+C (DDX21 Human)
    

    Tags

    GST-flag

    Expression

    Bacterial

    Mutation

    aa396-782

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. p23-mNeongreen-DDX21 HGV

    Plasmid

    #179230

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Tags

    mNeongreen

    Expression

    Mammalian

    Mutation

    D338H, E339G

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p23-mNeongreen-DDX21 DAT

    Plasmid

    #179229

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    DDX21 (DDX21 Human)
    

    Tags

    mNeongreen

    Expression

    Mammalian

    Mutation

    G234D, K235A

    Promoter

    EF-1alpha

    Available Since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. HIV-1-GFP ΔEnv CA E45A

    Plasmid

    #217438

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with E45A mutation in CA).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Mutation

    CA E45A

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. HIV-1-GFP ΔEnv CA Q63/67A

    Plasmid

    #217439

    Purpose

    HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu (with Q63A,Q67A mutations in CA).

    Depositor

    Jarrod Johnson

    Article

    Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

    Insert

    gag/pol
    

    Use

    Lentiviral

    Mutation

    CA Q63A,Q67A

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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