We narrowed to 15,736 results for: LEA

1. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

   Plasmid

   #203652

   Purpose

   Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Lentiviral

   Tags

   Sapphire-IRES-H2B-mCherry

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBW-m4133611

   Plasmid

   #152146

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 ORF7a protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF7a protein (ORF7a Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;HA

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pMAT11

   Plasmid

   #112592

   Purpose

   Used for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP.

   Depositor

   Marko Hyvönen, Johan Peränen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His-MBP

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. TRMT10C (RG9MTD1A-c113)

   Plasmid

   #110248

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   RG9MTD1 (TRMT10C Human)
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Promoter

   T7 - lacO

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1+ hnRNPK myc HIS

   Plasmid

   #71662

   Purpose

   Mammalian expression of hnRNPK

   Depositor

   Gideon Dreyfuss

   Insert

   hnRNPK (HNRNPK Human)
   

   Tags

   HIS and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_crGE

   Plasmid

   #172933

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSETA_crGE

   Plasmid

   #172927

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-Brainbow-2.1 R

   Plasmid

   #18723

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-Brainbow-1.1 M

   Plasmid

   #18722

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-Peredox-mCherry-NLS

    Plasmid

    #32384

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCherry-NLS
    

    Tags

    Nuclear Localization Seq

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. Thy1-Brainbow-1.1 M

    Plasmid

    #18726

    Depositor

    Joshua Sanes

    Article

    Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

    Insert

    Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
    

    Use

    Cre/Lox and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-Nsp14-TEV-Strep

    Plasmid

    #154953

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pSKB3.MPN348

    Plasmid

    #11437

    Depositor

    Sung-Hou Kim

    Article

    Chen et al Proteins. 2004 Sep 1. 56(4):839-43.

    Insert

    methenyltetrahydrofolate synthetase
    

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Available Since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-Brainbow-1.0 L

    Plasmid

    #18721

    Depositor

    Joshua Sanes

    Article

    Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

    Insert

    dTomato ; mCerulean ; EYFP
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. 23 pCOLI_A_N-SUMO_C_STREP

    Plasmid

    #160259

    Purpose

    N-term His-SUMO 3C cleavable, C--term 2xStrepII Amp resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    2xStrepII, 6xHis, and SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCoofy9

    Plasmid

    #122004

    Purpose

    E.coli vector for parallel SLIC cloning containing N-terminal StrepII with PreScission Cleavage site

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    StrepII

    Expression

    Bacterial

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. 5E26 (PANK2)

    Plasmid

    #79516

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PANK2 (PANK2 Human)
    

    Use

    Baculovirus expression

    Tags

    6xHis and TEV cleavage site

    Promoter

    polyhedrin

    Available Since

    June 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. mTagRFP-T2-PCNA-19

    Plasmid

    #58043

    Purpose

    Localization: Nucleus, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Insert

    PCNA (PCNA Human)
    

    Tags

    mTagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCoofy51

    Plasmid

    #122005

    Purpose

    Baculovirus vector for parallel SLIC cloning containing N-terminal TwinStrep with PreScission Cleavage site

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    TwinStrep

    Expression

    Insect

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. UHRF1

    Plasmid

    #28163

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    UHRF1 (UHRF1 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    tandem tudor domain

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits