We narrowed to 13,211 results for: CAR

1. pPRISM-Cre-cmlc2-eGFP

   Plasmid

   #117794

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   Cre
   

   Use

   Synthetic Biology

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-syn-FLEX-synaptoPAC-minWPRE

   Plasmid

   #153100

   Purpose

   Cre-conditional expression of synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

   Depositor

   Dietmar Schmitz

   Article

   Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

   Inserts

   bPAC(WT)
   Synaptophysin (Syp Rat)
   

   Use

   AAV

   Tags

   His tag, mScarlet, and myc tag

   Expression

   Mammalian

   Available Since

   Aug. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. fck-Jaws-GFP-ER2

   Plasmid

   #65013

   Purpose

   Lentivirus-mediated expression of Jaws-ER2 under the CaMKII promoter.

   Depositor

   Edward Boyden

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws-GFP-ER2
   

   Use

   Lentiviral

   Tags

   ER2 and GFP

   Expression

   Mammalian

   Mutation

   K200R W214F

   Promoter

   CaMKII

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTBL210

   Plasmid

   #126451

   Purpose

   Expresses Cas9-XTEN-TdT in mammalian cells.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   Cas9-XTEN-TdT (DNTT Human, Streptococcus pyogenes)
   

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   CMV

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPN458

   Plasmid

   #137876

   Purpose

   PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pATP416-­ptetO7-­pphlO6-­crtYBI

   Plasmid

   #165976

   Purpose

   Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Inserts

   Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
   Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
   BTS1 (BTS1 Budding Yeast)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   C1281A, G1698A and C66A, C1359T

   Promoter

   Synthetic promoter (pphlO6), Synthetic promoter (…

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGTag-TagRFP-B-actin

   Plasmid

   #117808

   Purpose

   Donor for precise CRISPR directed genomic integration using the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

   Insert

   TagRFP
   

   Use

   Synthetic Biology

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLRC1-NEK10p:NEK10-3XFLAG

   Plasmid

   #137030

   Purpose

   Lentiviral vector for expressing NEK10 under the control of the endogenous NEK10 promoter

   Depositor

   David Sabatini

   Article

   Chivukula et al Nat Med. 2020 Jan 20. pii: 10.1038/s4159

   Insert

   NIMA-like kinase 10 (NEK10 Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Promoter

   human NEK10 endogenous promoter

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. WT1 KI gRNA2

   Plasmid

   #92313

   Purpose

   CRISPR-GFP-gRNA for cutting WT1

   Depositor

   Sean Palecek

   Article

   Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

   Insert

   WT1 (WT1 Human)
   

   Tags

   CRISPR GFP

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 SARS-CoV-2 S D614G-trunc

    Plasmid

    #158077

    Purpose

    Expresses SARS-CoV-2 spike protein with a D614G truncated mutation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2 Halo-Tev- HSPB7 (zebrafish) WT

    Plasmid

    #108916

    Purpose

    Express WT Zebrafish Halo-Tev-HSPB7 in mammalian cells

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    Halo-TEV-HSPB7 (hspb7 Zebrafish)
    

    Tags

    Halo-TEV

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRepair-mScI-CTNNB1

    Plasmid

    #153431

    Purpose

    Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
    

    Use

    CRISPR; Hdr donor template

    Tags

    mScarlet-i

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE2-TetOminiCMV-OS

    Plasmid

    #136576

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Oct4, Sox2

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2 (Pou5f1, Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

    Plasmid

    #63885

    Purpose

    An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

    Insert

    ER-localized low-affinity GCaMP3(10.19)
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

    Plasmid

    #160623

    Purpose

    TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35S:dCas9:VPR-Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. INS-GFP-CD19-EPT

    Plasmid

    #210469

    Purpose

    Donor plasmid for knock-in GFP-CD19 into the human INS locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
    

    Use

    CRISPR

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPRISM-TagRFP-gcry-eGFP

    Plasmid

    #117767

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. XLone-Puro CCND2-T2A-luciferase-P2A-eGFP

    Plasmid

    #179843

    Purpose

    Tunable and temporal expression control of CCND2, luciferase and nuclear eGFP

    Depositor

    Xiaoping Bao

    Article

    Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Luciferase and Synthetic Biology

    Tags

    eGFP and luciferase

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

    Plasmid

    #114087

    Purpose

    U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for FnCas12a crRNAs
    

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR1A-HA-Jag1

    Plasmid

    #46052

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Jag1 (Jag1 Rat)
    

    Use

    Entry vector

    Tags

    HA tag

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only