We narrowed to 15,736 results for: LEA

1. pGBW-m4133371

   Plasmid

   #152780

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 helicase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;3xFlag

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. AD-003

   Plasmid

   #25502

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   AD-003 (NTMT1 Human)
   

   Tags

   6x His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

3. mEos3.2-LaminB1-10

   Plasmid

   #57464

   Purpose

   Localization: Nuclear Envelope, Excitation: 507 / 572, Emission: 516 / 580

   Depositor

   Michael Davidson

   Insert

   LaminB1 (LMNB1 Human)
   

   Tags

   mEos3.2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pGBW-m4133317

   Plasmid

   #152313

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;3xFlag

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   Sept. 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGBW-m4134093

   Plasmid

   #152116

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;V5

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. mPlum-Fibrillarin-7

   Plasmid

   #55969

   Purpose

   Localization: Nuclear - Nucleolus, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Insert

   Fibrillarin (FBL Human)
   

   Tags

   mPlum

   Expression

   Mammalian

   Mutation

   R36Q, S66F

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pGBW-m4134157

   Plasmid

   #152062

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 nsp6

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;HA

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   May 19, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pGBW-m4133850

   Plasmid

   #152865

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;3xFlag

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   July 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. 4QPP

   Plasmid

   #61964

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HRMT1L6:PBC001-A12:C213642 (PRMT6 Human)
   

   Use

   Baculovirus expression

   Tags

   6x His and TEV cleavage site

   Mutation

   See comments

   Promoter

   Polyhedrin

   Available Since

   Sept. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pNIC-PelB-NH10T-AVITAG-GG

    Plasmid

    #215793

    Purpose

    Cloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pGBW-m4133424

    Plasmid

    #152851

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    June 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pGBW-m4134229

    Plasmid

    #152097

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pGBW-m4134397

    Plasmid

    #152706

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pBXC3H

    Plasmid

    #47068

    Purpose

    FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site) and decaHis (…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid

    #174902

    Purpose

    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Tags

    FLAG and NES

    Expression

    Mammalian

    Mutation

    Y306A, Y384F

    Promoter

    EF1

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. p7XC3GH

    Plasmid

    #47066

    Purpose

    FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBW-m4133643

    Plasmid

    #152361

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4134063

    Plasmid

    #152631

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4134251

    Plasmid

    #152087

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4134375

    Plasmid

    #152788

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge