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PMT-mCherry2_2

Plasmid

#203784

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Publication

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mCherry2 dimer

Expression

Mammalian

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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PMT-mScarlet_2

Plasmid

#203780

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Publication

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mScarlet dimer

Expression

Mammalian

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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PMT-mApple_2

Plasmid

#203772

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Publication

Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

Insert

PMT-mApple dimer

Expression

Mammalian

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-PIP5K1BD266K

Plasmid

#202723

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1B(D266K) (PIP5K1B Human)

Expression

Mammalian

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSF-NgPglO

Plasmid

#198204

Purpose

pSN18 derivative encoding N. gonorrhoeae PglO with C-terminal FLAG epitope tag

Depositor

Michael Jewett

Publication

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

NgPglO

Tags

Flag tag

Expression

Bacterial

Promoter

pBAD

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-PD-DQNAT

Plasmid

#198216

Purpose

pJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Publication

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

PD-DQNAT

Tags

6x His Tag and DQNAT glycosylation tag

Expression

Bacterial

Promoter

T7

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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SpVasa-S83K-GFP

Plasmid

#185588

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-S83K

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

S83K

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-E441Q-GFP

Plasmid

#185590

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-E441Q

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

E441Q

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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p09008_AF1-4+GFP5-7

Plasmid

#173715

Purpose

Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

Depositor

Johannes Kabisch

Publication

Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

Inserts

hybrid GFP
mKate2

Use

E. coli cell free protein synthesis extracts

Expression

Bacterial

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE010-sgRNA-Target1 (MpRTN1)

Plasmid

#186295

Purpose

Binary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Publication

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Expression

Bacterial

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mTurbo-mito

Plasmid

#187177

Purpose

Express mitochondira-localized miniTurbo under the control of CAG promoter

Depositor

Isei Tanida

Publication

Sanada et al Sci Rep. 2022 Jul 1;12(1):11130. doi: 10

Insert

mtActA fused with miniTurbo (BirA mutant)

Expression

Mammalian

Promoter

CAG

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-SpBeta-catenin

Plasmid

#185579

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Publication

Wavreil et al Dev Biol. 2021 Oct;478:13-24. doi: 10.10

Insert

Beta-catenin

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-K332N-GFP

Plasmid

#185592

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-K332N

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

K332N

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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SpVasa-S636K-GFP

Plasmid

#185589

Purpose

to see protein dynamics

Depositor

Mamiko Yajima

Publication

Fernandez-Nicolas et al Dev Dyn. 2019 Dec;248(12):1273-1285. doi

Insert

Vasa-S636K

Use

In vitro transcription (mrna synthesis)

Tags

GFP

Mutation

S636K

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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C453-E26: attB4-T7p>-attB5

Plasmid

#162982

Purpose

Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

bacteriophage T7 promoter

Use

Synthetic Biology

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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C231-E02: attB2r-NanoLuc-attB3

Plasmid

#162901

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal NanoLuc fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Nanoluciferase

Use

Synthetic Biology

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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C121-E06: gLuc

Plasmid

#162890

Purpose

Standard Gateway entry clone encoding Gaussia luciferase

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Gaussia princeps luciferase

Use

Synthetic Biology

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-313 (attR4-attR3)

Plasmid

#161898

Purpose

Multisite Gateway (attR4-attR3) vector for mammalian protein expression

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Mammalian

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-CaaX(tH) in pCSDEST2

Plasmid

#140890

Purpose

Expression of GFP-CaaX (sequence from Hras) in mammalian cells.

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX membrane targeting motif (H-ras)

Tags

EGFP

Expression

Mammalian

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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S3_007_026_MV1

Plasmid

#134419

Purpose

pYTKMV1-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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