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DJ-L1 gRNA #5

Plasmid

#247545

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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DJ-L1 gRNA #4

Plasmid

#247544

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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DJ-L1 gRNA #3

Plasmid

#247543

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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DJ-L1 gRNA #1

Plasmid

#247542

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_TIMM23_Puro_T2A_BFP2

Plasmid

#236731

Purpose

Dual sgRNA targeting TIMM23 expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_TIMM23_ER-dAPEX-BFP2

Plasmid

#236734

Purpose

Dual sgRNA targeting TIMM23 expressing dAPEX-BFP targeting to ER

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Neo1 gRNA

Plasmid

#246575

Purpose

CRISPR vector co-expressing Cas9 and a mouse Neo1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Neo1 gRNA (Neo1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Nrp1 gRNA

Plasmid

#246578

Purpose

CRISPR vector co-expressing Cas9 and a mouse Nrp1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Nrp1 gRNA (Nrp1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Plxna1 gRNA

Plasmid

#246574

Purpose

CRISPR vector co-expressing Cas9 and a mouse Plxna1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Plxna1 gRNA (Plxna1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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His-Thrombin-Munc13-1 C1-C2B-MUN-C2C (aa529-1735 delta1408-1452 delta1533-1551)

Plasmid

#243821

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Article

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

His

Expression

Bacterial

Mutation

delta1408-1452 delta1533-1551

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn1-mCherry-CAAX

Plasmid

#223674

Purpose

AAV expression of mCherry from hSyn1 promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mCherry-CAAX

Use

AAV

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-hDPPIII

Plasmid

#233510

Purpose

Mammalian expression vector

Depositor

Shyam Chauhan

Article

Prajapati et al Biol Chem. 2016 Jun 1;397(6):563-9. doi:

Insert

hDPPIII (DPP3 Human)

Expression

Mammalian

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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His-Thrombin-Munc13-1 C1-C2B-MUN-C2C R1598E F1658E (delta1408-1452 delta1533-1551)

Plasmid

#243822

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Article

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

His

Expression

Bacterial

Mutation

delta1408-1452 delta1533-1551 R1598E F1658E

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Integrin β3-EBFP2

Plasmid

#242827

Purpose

Encodes wildtype human integrin β3 and C-terminal EBFP2 tag

Depositor

Cheng-han Yu

Article

Lee et al Adv Sci (Weinh). 2025 Jun 10:e01078. doi

Insert

Integrin subunit beta 3 (ITGB3 Human)

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET50del_SynPspA_R44K/E126Q/E179Q

Plasmid

#223819

Purpose

Bacterial expression of SynPspA R44K/E126Q/E179Q with N-terminal 6xHis-Tag and 3C cleavage site

Depositor

Carsten Sachse

Article

Junglas et al Nat Struct Mol Biol. 2025 Jan;32(1):23-3

Insert

SynPspA

Tags

6xHis

Expression

Bacterial

Mutation

R44K, E126Q, E179Q

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-GalK-USP-ET64

Plasmid

#234662

Purpose

Expression an ELP-tagged fusion enzyme of GalK and AtUSP

Depositor

Xianwei Liu

Article

Wu et al Chembiochem. 2025 Jan 13:e202401005. doi

Inserts

galactokinase
UDP-sugar pyrophosphorylase (USP Mustard Weed)

Tags

ELP

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1625

Plasmid

#239834

Purpose

OrufIscB-REC E. coli expression for recombinant purification

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB-REC

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Mutation

Insertion of REC domain from NbaCas9-1

Promoter

T7

Available Since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pHS1505

Plasmid

#239830

Purpose

OrufIscB-REC mammalian expression

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

OrufIscB-REC

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Mutation

Insertion of REC domain from NbaCas9-1

Promoter

CMV

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pYO539

Plasmid

#235752

Purpose

Protein expression of ScVPS30 and ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Expression

Yeast

Promoter

GAL-TDH3

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1alpha TDH

Plasmid

#236457

Purpose

stable expression of TDH using viral infection

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

TDH (Tdh Mouse)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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