We narrowed to 17,800 results for: Jun
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Plasmid#147860PurposeInsect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAresDepositorInsertDmNot1-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)
ExpressionInsectMutation10 silent mutations and two non silent N605S, K75…Available SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_812-1945-dsRNAres_W
Plasmid#147845PurposeInsect Expression of DmNot1_812-1945-dsRNAresDepositorInsertDmNot1_812-1945-dsRNAres (Not1 Fly)
ExpressionInsectMutation3 silent mutations compared to the sequence given…Available SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-HsNot1iso1_1356-1581_V
Plasmid#147779PurposeBacterial Expression of HsNot1iso1_1356-1581DepositorInsertHsNot1iso1_1356-1581 (CNOT1 Human)
ExpressionBacterialAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_296-472-10xmut_AC
Plasmid#148420PurposeMammalian Expression of HsRck_296-472-10xmutDepositorInsertHsRck_296-472-10xmut (DDX6 Human)
ExpressionMammalianMutationQ309A, H312A, T316A, R320A, Q322A, N324A, R335A, …Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry
Plasmid#179434PurposeDual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertTRE-cit-HS4-mch (SH)
ExpressionMammalianPromoterpEF alpha, pRSVAvailable SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F594V-siRNAres_F
Plasmid#146331PurposeInsect Expression of DmAGO1-F594VDepositorInsertDmAGO1-F594V (AGO1 Fly)
ExpressionInsectMutationvariation with 2 non silent mutations compared to…Available SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL006_phiC31_neo-5xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry
Plasmid#179425PurposeDual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integrationDepositorInsertTRE-cit-(nospacer)-mch
ExpressionMammalianPromoterpEF alpha, pRSVAvailable SinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo2 mycBioID-Tollip M240A,F241A
Plasmid#178055Purposemyc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.DepositorInsertToll-Interacting Protein (TOLLIP Human)
TagsmycBioIDExpressionMammalianMutationChanged Methionine 240 to Alanine and changed Phe…PromoterCMVAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR207-MUM2sp-Citrine-ADPG2
Plasmid#170725PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)DepositorInsertARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
UseGateway donor vector / entry cloneTagsCitrine tag (714bp)Available SinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUFlip-floxed2A-KalTA4-; gcry1:BFP -2
Plasmid#173891PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; gcry1: BFP
UseSynthetic BiologyAvailable SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔEC gRNA resistant
Plasmid#163413PurposeExpresses BMPR-2ΔEC resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
ExpressionMammalianMutationThe extracellular domain (27-150 aa) is deleted. …Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Bmpr2ΔTail gRNA resistant
Plasmid#163412PurposeExpresses BMPR-2ΔTail resistant to the gRNAs(#163388-90) in mammalian cellsDepositorInsertBmpr2 (Bmpr2 Mouse)
ExpressionMammalianMutationThe C-terminal tail domain (530-1038 aa) is delet…Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQlinkG2-PTPIP51(236-470)
Plasmid#170534PurposeExpresses Cleavable GST-tagged PTPIP51 TPR domain (236-470) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 Human)
TagsGST tagExpressionBacterialPromoterT5 promoterAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFASTbac-HisGST-PTPIP51(36-470)
Plasmid#170531PurposeExpresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9DepositorInsertProtein tyrosine phosphatase interacting protein 51 (RMDN3 )
TagsGST tag and His tagExpressionInsectPromoterPolyhedrinAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQlinkH-PTPIP51(236-470)
Plasmid#170530PurposeExpresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. ColiDepositorInsertProtein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)
TagsHis tagExpressionBacterialPromoterT5 promoterAvailable SinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42 -SSRP1
Plasmid#170439PurposeExpress drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.DepositorAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSico_U6-PLOD2 sgRNA3
Plasmid#136458PurposeExpression of gRNA against human PLOD2DepositorInsertgRNA against human PLOD2 (PLOD2 Synthetic, Human)
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSico_U6-PLOD2 sgRNA5
Plasmid#136460PurposeExpression of gRNA against human PLOD2DepositorInsertgRNA against human PLOD2 (PLOD2 Synthetic, Human)
UseCRISPR and LentiviralExpressionMammalianAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Direct-seq_lentiGuide-Puro-Tetra Loop-8A8G
Plasmid#157982PurposeExpresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.DepositorInsertS. pyogenes sgRNA cassette (Tetra-8A8G)
UseCRISPR and LentiviralExpressionMammalianPromoterhU6Available SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJV126
Plasmid#124250PurposeIn vitro transcription plasmid for T7 transcription of wildtype Her2 sequence targeted to the endosome. Linearize with SapI.DepositorInsertMinigene containing Her2 sequences fused to endosomal targeting sequence of class I MHC
UseIn vitro transcription templatePromoterT7Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only