We narrowed to 7,912 results for: Lif
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Plasmid#172838PurposeMammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-VPS34
Plasmid#99327PurposeComponent for mammalian expression of recombinant PI3KC3-C1 complexDepositorAvailable SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3α-AS4-
Plasmid#184332PurposeExpression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.DepositorAvailable SinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::T48-Baz::vhhGFP4
Plasmid#163928PurposeExpression of GrabFP-A-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with T48 protein, Bazooka minimal localization sequence and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-2B-Tbx5
Plasmid#98619PurposeGateway entry vector TBX5DepositorInsertTBX5 (Tbx5 Mouse)
UseGateway CloningAvailable SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR
Plasmid#67931PurposeTranslational reporter - ARHGAP5 UTRsDepositorAvailable SinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3α-AS4+
Plasmid#184333PurposeExpression of mouse Nrx3-alpha containing insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.DepositorAvailable SinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLEXm-GST-ATG14
Plasmid#99329PurposeComponent for mammalian expression of recombinant PI3KC3-C1 complexDepositorInsertATG14 (ATG14 Human)
TagsGST-TEV tagExpressionMammalianMutationCodon optimizedPromoterCMVAvailable SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330 _CRISPIE donor B10
Plasmid#172851PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B10 (Zhong et al, eLife 2021), mEGFP translational phase (2-stop), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-stop) encoding mEGFP
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330_Actb sgRNA / hSpCas9
Plasmid#172832PurposeMammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only