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EGFP-CaaX(tH) in pCSDEST2

Plasmid

#140890

Purpose

Expression of GFP-CaaX (sequence from Hras) in mammalian cells.

Depositor

Rob Parton

Publication

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX membrane targeting motif (H-ras)

Tags

EGFP

Expression

Mammalian

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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S3_007_026_MV1

Plasmid

#134419

Purpose

pYTKMV1-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_008_026_MV1

Plasmid

#134420

Purpose

pYTKMV1-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pRPL18B

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_009_026_MV1

Plasmid

#134421

Purpose

pYTKMV1-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pREV1

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_007_026_MV2

Plasmid

#134424

Purpose

pYTKMV2-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_007_026_096

Plasmid

#134410

Purpose

pYTK096-pTDH3_ NLS_FdeR-N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pTDH3

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_008_026_096

Plasmid

#134411

Purpose

pYTK096-pRPL18B_ NLS_FdeR_N_2NLS _tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pRPL18B

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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S3_009_026_096

Plasmid

#134412

Purpose

pYTK096-pREV1_ NLS_FdeR-N_2NLS-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
NLS_FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pREV1

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S3_004_026_096

Plasmid

#134407

Purpose

pYTK096-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pTDH3

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S3_006_026_096

Plasmid

#134409

Purpose

pYTK096-pREV1_FdeR-N_2NLS_tPGK1- pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pREV1

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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S3_006_026_096

Plasmid

#134418

Purpose

pYTK096-pREV1_FdeR-N_2NLS_tPGK1- pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pREV1

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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S3_004_026_MV2

Plasmid

#134423

Purpose

pYTKMV2-pTDH3_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pTDH3

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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S3_005_026_MV1

Plasmid

#134417

Purpose

pYTKMV1-pRPL18B_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGMP1 and pRPL18B

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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S3_005_026_096

Plasmid

#134408

Purpose

pYTK096-pRPL18B_FdeR-N_2NLS_tPGK1-pGPM1-fdeO_mcherry_tTDH1

Depositor

Mattheos Koffas

Publication

Wang et al ACS Synth Biol. 2019 Sep 20;8(9):2121-21

Inserts

mCherry
FdeR-N_2xNLS

Tags

none

Expression

Yeast

Mutation

none

Promoter

pGPM1 and pRPL18B

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI119

Plasmid

#126059

Purpose

Module 0 vector of PlaMiNGo toolkit

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

chloroplastic FNR transit peptide

Use

Synthetic Biology; Module 0

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG082

Plasmid

#114000

Purpose

Level 0 module. NifH promoter for nodule-specific expression

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

NifH promoter

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YIplac204-iGFP-YPT52

Plasmid

#105279

Purpose

Plasmid for integration of iGFP-Ypt52 at TRP1.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Ypt52 (YPT52 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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YCe3777 HC_Kan_Chimeric-5'LTR_p1

Plasmid

#100704

Purpose

part designed to occupy position 1 of EMMA. Functional category: Long Terminal Repeat

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Lentiviral chimeric 5' LTR

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2030 HC_Kan_bGHpA_p16

Plasmid

#100688

Purpose

part designed to occupy position 16 of EMMA. Functional category: polyadenylation signal sequence

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

bGHpA

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2058 HC_Kan_Kozak_ATG_Palm_p6

Plasmid

#100694

Purpose

part designed to occupy position 6 of EMMA. Functional category: Signal peptide for subcellular targeting

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Kozak_ATG_Palm

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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