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A Bacterial Expression Vector Archive (BEVA) for Flexible Modular Assembly of Golden Gate-Compatible Vectors.
Geddes BA, Mendoza-Suarez MA, Poole PS
Front Microbiol. 2019 Jan 14;9:3345. doi: 10.3389/fmicb.2018.03345. eCollection 2018.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
113978pOGG001Level 0 module. nptII promoter for constitutive gene expression
113979pOGG004Vector construction module. Level 1 golden gate cloning site module with LacZ reporter
113980pOGG005Level 1 high copy number vector for E. coli expression
113981pOGG006Golden Gate Level 2 cloning site, position 1 module for vector construction
113982pOGG008Vector construction module. Kanamycin/neomycin resistance module for construction of Golden Gate cloning vectors
113983pOGG009Vector construction module. Gentamicin resistance module for construction of Golden Gate cloning vectors
113984pOGG010Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors
113985pOGG011Vector construction module. pBBR1 medium-copy number origin of replication for construction of Golden Gate cloning vectors
113986pOGG012Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors
113987pOGG013Vector construction module. Endlinker/terminator module to circularize plasmid following Part 3
113988pOGG014Vector construction module. Endlinker/terminator module to circularize plasmid following Part 4
113989pOGG015Vector construction module. Endlinker/terminator module to circularize plasmid following Part 5
113990pOGG016Vector construction module. Endlinker/terminator module to circularize plasmid following Part 6
113991pOGG024Level 1 golden gate cloning vector for medium copy number free-living expression
113992pOGG026Level 1 golden gate cloning vector for low copy number stable environmental gene expression
113993pOGG030Level 0 module. IPTG-inducible T7RNAP promoter for BL21 protein purification
113994pOGG031Level 0 module. IPTG promoter for inducible expression
113995pOGG037SC module. Super-folder GFP green fluorescence reporter
113996pOGG039SC module. Terminator for T7RNAP
113997pOGG041Level 0 module. Taurine-inducible promoter for Alphaproteobacteria
113998pOGG042Vector construction module. Tetracycline resistance module for construction of Golden Gate cloning vectors
113999pOGG050SC module. Thermostable beta-galactosidase reporter
114000pOGG082Level 0 module. NifH promoter for nodule-specific expression
114001pOGG083SC module. Beta-glucosidase reporter
114002pOGG216Level 2 golden gate cloning vector for medium copy number free-living expression
115503pOGG202Clone of a level 1 unit which encodes IPTG-inducible sfGFP
115504pOGG203Clone of a level 1 unit which encodes a constitutively-expressed mCherry
115505pOPS0253Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.
115506pOPS0254Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celB
115507pOPS0314Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB
115508pOPS0359 Reporter Plasmid. Taurine-inducible expression of GFP
115509pOPS0377Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP
115510pOPS0379Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP
115511pOPS0750Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.

Antibodies from Article