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px330 CBP gRNA

Plasmid

#165587

Purpose

insertin of CBP degrom

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

CBP (Crebbp Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AMPKsub-YPet-Kras

Plasmid

#84638

Purpose

Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); plasma membrane targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

AMPKsub-YPet-Kras

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBABEhygro-Flag-ZO-2-1st-PDZ-mut

Plasmid

#27474

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

K38E, F44H, I46E and V48E

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5 HA-CCDC95

Plasmid

#19795

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

Insert

CCDC95 (INO80E Human)

Tags

2XHA

Expression

Mammalian

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pKD265

Plasmid

#125095

Purpose

Expression of torR(1-134aa)-psdR(134-237aa), ompR137 chimera under PLtetO-1, output Promoter PpsdA110

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

torR(REC)-psdR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSR322

Plasmid

#125116

Purpose

Expression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

uhpA(REC)-ydfI(DBD)131
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.15

Plasmid

#125082

Purpose

Expression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.29

Plasmid

#125088

Purpose

Expression of ompR(1-139aa)-ccaR(129-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)139
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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5' Del 3

Plasmid

#16051

Depositor

Robert Benezra

Article

Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

Insert

Id1 promoter, 5' deletion (Id1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

-927 to +88 of promoter

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pDECKO-mCherry TFRC_B

Plasmid

#78544

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock V315R

Plasmid

#47342

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

V315R

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCMV10/3xflag-Clock W284A

Plasmid

#47341

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

W284A

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Ywhag-3'UTR-MRE-3 MUT

Plasmid

#61792

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhag with MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhag 3'UTR with miR-200c MRE-3 mutated

Use

Luciferase

Mutation

miR-200c MRE-3 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pLacIBD::SDRX (GB0673)

Plasmid

#68183

Purpose

Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

LacI Binding Domain fused to SRDX Transcriptional Repressor

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_NOSTRIN-1/1-SV1/2

Plasmid

#91323

Purpose

Protein expression and purification of human SH3 domain construct NOSTRIN-1/1-SV1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NOSTRIN-1/1-SV1/2 (NOSTRIN Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMX-Flag-WIP 460 deletion

Plasmid

#11773

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WIP aa1-460 (WIPF1 Human)

Use

Retroviral

Tags

flag

Expression

Mammalian

Mutation

aa461-503 deleted

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
HOXA4 (murine) pET

Plasmid

#8488

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXA4 (Hoxa4 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

Thr-2 changed to Ala-2 to form better ribosomal b…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
p2Flag CMV2-ZO-2-delta-N

Plasmid

#27419

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

2xFlag

Expression

Mammalian

Mutation

Deletion of coding nucleotides from 1-351. This r…

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only
pHH0103_SH3KBP1-1/3

Plasmid

#91555

Purpose

Protein expression and purification of human SH3 domain construct SH3KBP1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3KBP1-1/3 (SH3KBP1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc NES- mCE(K294A)

Plasmid

#82464

Purpose

Expresses myc tag and catalytic inactive form of mRNA capping enzyme with N terminal Nuclear export signal (NES)

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NES mRNA capping enzyme (Rngtt Mouse, HIV)

Tags

myc

Expression

Mammalian

Mutation

K294A

Promoter

CMV

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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