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pAM356 pET28a-GGG-Titin[V15P]-23-K-29-C-5

Plasmid

#226352

Purpose

Expression of Titin (V15P) substrate for 26S proteasome

Depositor

Andreas Martin

Article

Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

Insert

titin (TTN Human)

Expression

Bacterial

Mutation

V15P

Promoter

T7

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAM357 pET28a-GGG-Titin[V15P]-23-K-33-C-1

Plasmid

#226353

Purpose

Expression of titin (V15P) substrate for 26S proteasome

Depositor

Andreas Martin

Article

Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

Insert

titin (TTN Human)

Expression

Bacterial

Mutation

V15P

Promoter

T7

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-N1-KH12mut (T236D H237D K299D N300D)

Plasmid

#225454

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (T236D H237D K299D N300D)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

T236D H237D K299D N300D

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanIV-Chondromyces

Plasmid

#225078

Purpose

Expresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIV-Chondromyces)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanI-101

Plasmid

#225065

Purpose

Expresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanI-101)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML RING

Plasmid

#185631

Purpose

expression of GST-tagged PML RING domain in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Structure. 2023 Sep 7;31(9):1086-1099.e6

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-PML RB1

Plasmid

#185636

Purpose

expression of GST-tagged PML fragment 49-168 (RING + B-box1) in bacterial cells

Depositor

Irina Bezsonova

Article

Bregnard et al Biophys Chem. 2022 Aug;287:106827. doi:

Insert

promyelocytic leukemia protein (PML Human)

Tags

GST

Expression

Bacterial

Mutation

none

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGFP-mVprBP pc2953

Plasmid

#201698

Purpose

Expresses of GFP-tagged VprBP in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

VprBP (Dcaf1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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pmcherry-mVprBP pc2954

Plasmid

#201699

Purpose

Expresses of mcherry-tagged mVprBP in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

VprBP (Dcaf1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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Ubc-c004

Plasmid

#216875

Purpose

Bacterial expression vector for E. coli producing ubiquitin with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

Insert

Ubiquitin (UBC Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265E7

Plasmid

#213470

Purpose

Gateway entry clone (attL1 & attR5) TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadA-CDd-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tada-cdd-32aa linker-z…

Tags

No

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265E8

Plasmid

#213471

Purpose

Gateway entry clone (attL1 & attR5) TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadA-CDd_V106W-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tada-cdd_v106w-32aa li…

Tags

No

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7578 pHR (hU6-crPROX-EFS-PuroR-WPRE)

Plasmid

#214882

Purpose

Lentiviral vector encoding RfxCas13d targeting PROX guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crPROX-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-SRRD-mRuby3

Plasmid

#214920

Purpose

inducible expression of SRRD tagged on C-terminus with mRuby3

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

mRuby3

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-noTag (in pLEX_307)

Plasmid

#214919

Purpose

constitutive expression of SRRD with no tag - used as control for SRRD-HA expression

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-SRRD-HA (in pLEX_307)

Plasmid

#214918

Purpose

constitutive expression of SRRD tagged with HA

Depositor

Ophir Shalem

Article

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

SRRD (SRRD Human)

Use

Lentiviral

Tags

HA

Available Since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-deltaExon17

Plasmid

#209563

Purpose

Mammalian expression of Shtn1 long isoform with E17 deletion

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with E17 deletion (4930506M07Rik Mouse)

Expression

Mammalian

Mutation

Exon17 deletion

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-SHTN1LRRR>GGG

Plasmid

#209562

Purpose

Mammalian expression of Shtn1 long isoform with RG mutation

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with RG mutation (4930506M07Rik Mouse)

Expression

Mammalian

Mutation

RRR to GGG

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-deltaExon16-17

Plasmid

#209564

Purpose

Mammalian expression of Shtn1 long isoform with E16-17 deletion

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with E16-17 deletion (4930506M07Rik Mouse)

Expression

Mammalian

Mutation

Exon16 and 17 deletion

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-deltaExon15-II

Plasmid

#209566

Purpose

Mammalian expression of Shtn1 long isoform with E15 deletion II

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with E15 deletion II (4930506M07Rik Mouse)

Expression

Mammalian

Mutation

Exon15 deletion II

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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