We narrowed to 16,131 results for: form

1. pMYC BRGI aa1400-1648

   Plasmid

   #19145

   Depositor

   Joan Massague

   Article

   Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   human BRG1 aa 1400-1648 (PCR product)

   Available Since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pEGFP-C1 hSlp2-a C2AB

   Plasmid

   #40051

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   hSlp2-a C2AB (SYTL2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C2AB domains only (aa 597-910)

   Promoter

   CMV promoter

   Available Since

   Oct. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1 (-) hYAP 1-2gamma

   Plasmid

   #124145

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Megan Finch-Edmondson, Marius Sudol

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Mutation

   None (Wild Type)

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIE-C-hα2M-H6

   Plasmid

   #128494

   Purpose

   Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (R787-N1473) (A2M Human)
   

   Tags

   His tag (6x)

   Expression

   Insect

   Mutation

   contains R787-N1473

   Promoter

   IE1

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti PGK Puro GFP-GABARAPL1 G116A

   Plasmid

   #123245

   Purpose

   Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Glycine 116 to Alanine

   Promoter

   PGK

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTWIN1-His6-Ssp-Httex1-29Q

   Plasmid

   #84350

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 29Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Tags

   N-terminal Ssp intein (His-tagged)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCHKO_intergenic-intergenic_1

   Plasmid

   #155077

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

   Depositor

   Benjamin Blencowe, Jason Moffat

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET29-hMIGA2(306-593)-6xhis

   Plasmid

   #192837

   Purpose

   Express codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 (-) hYAP 1-1alpha

   Plasmid

   #124139

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Megan Finch-Edmondson, Marius Sudol

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Mutation

   None (Wild Type)

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTWIN1-His6-Ssp-Httex1-37Q

    Plasmid

    #84351

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 37Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon 1 (HTT Human)
    

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Rufy3 scFv [N483/26]

    Plasmid

    #190558

    Purpose

    Mammalian Expression of Rufy3 scFV. Derived from hybridoma N483/26.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Rufy3 (Mus musculus) recombinant scFV (Rufy3 Mouse)
    

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pATP416-­pluxO5-­ptetO7-­crtYBI

    Plasmid

    #165975

    Purpose

    Expresses carotenoid biosynthesis gene in response to homoserine lactone and doxycycline in yeast expressing LuxTA and rtTA

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Inserts

    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Mutation

    C1281A, G1698A and C66A, C1359T

    Promoter

    Synthetic promoter (pluxO5), Synthetic promoter (…

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. TrpV1 scFv [N221/17]

    Plasmid

    #190540

    Purpose

    Mammalian Expression of TrpV1 scFV. Derived from hybridoma N221/17.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    TrpV1 (Rattus norvegicus) recombinant scFV (Trpv1 Mouse)
    

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTWIN1-His6-Ssp-Httex1-15Q

    Plasmid

    #84348

    Purpose

    Expression of the human Huntingtin Exon1 protein containing 15Q in E.coli

    Depositor

    Hilal Lashuel

    Article

    Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Tags

    N-terminal Ssp intein (His-tagged)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. FB013

    Plasmid

    #119710

    Purpose

    TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 Flag MFF iso5 S172A

    Plasmid

    #74382

    Purpose

    Flag tagged human S172A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser 172 Ala (numbering based on MFF isoform 1)

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCDNA3 Flag MFF iso5 S155,172A

    Plasmid

    #74383

    Purpose

    Flag tagged human S155,172A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    See 155 Ala, Ser 172 Ala (numbering based on MFF …

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pBOB-N-3HA-GK_v1

    Plasmid

    #134275

    Purpose

    Lentivector encoding 3XHA-tagged GK (isoform 1)

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    Gk (Gk Mouse)
    

    Use

    Lentiviral

    Tags

    3X HA

    Expression

    Mammalian

    Mutation

    524 amino acids, isoform 1

    Promoter

    CMV

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLCHKO_TK1_HPRT1_Lb_4

    Plasmid

    #155062

    Purpose

    Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE (hygro) hYAP 1-1delta

    Plasmid

    #124150

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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