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TV_EMPTY-ires-mNeon-nls-p2a-dtr-wpre_pgk-puro

Plasmid

#251174

Purpose

Empty targeting vector (SapI sites intact) for C-terminal knockin at desired locus to enable reporting of expression by mNeonGreen and depletion of positive cells with DTR

Depositor

Hugo Snippert

Insert

double SapI flanked ires-mNeon-nls-p2a-dtr-wpre_pgk-puro

Use

CRISPR

Tags

ires-mNeon-nls-p2a-dtr

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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HRPEadh-pAG303

Plasmid

#249735

Purpose

Derived from pAG303GPD-ccdb (#14134); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs.

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pLVX-CAG-MCS

Plasmid

#225209

Purpose

Lentiviral vector with CAG promoter and multiple cloning site

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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HRPEadh-pAG305

Plasmid

#249734

Purpose

Derived from pAG305GPD-ccdb (#14138); GPD replaced with a synthetic 5xHRPE-ADHutr sequence for orthogonal activation by plant RAP2.12-based TFs.

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_(3'UTR)_RLEloop

Plasmid

#240253

Purpose

PROTEUS vector with no transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAG304-ccdbNLUC

Plasmid

#249736

Purpose

Derived from pAG304GPD-ccdb (#14136); GPD removed and NLUC cds inserted downstream gateway cassette. Useful for cloning promoter-NLUC fusions in Saccharomyces cerevisiae

Depositor

Francesco Licausi

Article

Lavilla-Puerta et al bioRxiv 2025.09.01.673502

Type

Empty backbone

Expression

Yeast

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pAKanCRISPR

Plasmid

#242231

Purpose

A sgRNA expression plasmid for genome editing in Pseudomonas

Depositor

James T. Tambong

Article

Chi et al Sci Rep. 2024 Sep 3;14(1):20517. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pART27

Plasmid

#247986

Purpose

Binary vector to receive a NotI fragment from pART7 with a gene to be stably expressed in plants via agrobacterium-mediated transformation.

Depositor

Andrew Gleave, Christoph Plieth

Article

Gleave et al Plant Mol Biol. 1992 Dec;20(6):1203-7.

Type

Empty backbone

Expression

Plant

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pYSGDLuc

Plasmid

#236297

Purpose

Dual luciferase reporter for measuring recoding efficiencies in yeast

Depositor

Pasha Baranov

Article

Fenton et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Expression

Yeast

Promoter

TEF1

Available Since

Dec. 1, 2025

Availability

Academic Institutions and Nonprofits only

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EML8357_pA3A-CBECas9

Plasmid

#234352

Purpose

A base editor derived by egg-cell specific promoter

Depositor

Erh-Min Lai

Article

Liu et al New Phytol. 2025 Nov 30. doi: 10.1111/np

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Nov. 25, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-rtTA-2A-tagBFP-bGH_pKG3264

Plasmid

#246372

Purpose

Expression plasmid for rtTA-2A-tagBFP driven from the CAG promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

rtTA

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-rtTA-2A-mRuby2-bGH_pKG2064

Plasmid

#246373

Purpose

Expression plasmid for rtTA-2A-mRuby2 driven from the EFS promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

rtTA

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_3C_mVenus_FR

Plasmid

#240669

Purpose

Expression plasmid for 3C-mVenus-tagged proteins with FAST-Red transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

3C-mVenus

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_FG

Plasmid

#240670

Purpose

Expression plasmid for un-tagged proteins with FAST-Green transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_TEV_HA_FG

Plasmid

#240677

Purpose

Expression plasmid for TEV-HA-tagged proteins with FAST-Green transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-HA

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_3C_mVenus_FG

Plasmid

#240679

Purpose

Expression plasmid for 3C-mVenus-tagged proteins with FAST-Green transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

3C-mVenus

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_TEV_mCherry_Hyg

Plasmid

#240652

Purpose

Expression plasmid for TEV-mCherry-tagged proteins with Hygromycin transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-mCherry

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_TEV_2xStrepII_Hyg

Plasmid

#240655

Purpose

Expression plasmid for TEV-2xStrepII-tagged proteins with Hygromycin transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-2xStrepII

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_TEV_MYC_Hyg

Plasmid

#240656

Purpose

Expression plasmid for TEV-MYC-tagged proteins with Hygromycin transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-MYC

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_TEV_HA_Hyg

Plasmid

#240657

Purpose

Expression plasmid for TEV-HA-tagged proteins with Hygromycin transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-HA

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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