Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,673 results for: pcr

Clear keywords
Showing: 921 - 940 of 4673 results

  1. PCRII-zSynA

    Plasmid
    #20121
    Depositor
    Aaron Gitler
    Insert
    zSynA (sncgb Zebrafish)
    Use
    In-situ
    Available since
    Jan. 20, 2009
    Availability
    Academic Institutions and Nonprofits only

  2. PCRII-zSynB

    Plasmid
    #20122
    Depositor
    Aaron Gitler
    Insert
    zSynB (sncga Zebrafish)
    Use
    In-situ
    Available since
    Jan. 9, 2009
    Availability
    Academic Institutions and Nonprofits only

  6. pEasyG2_mic

    Plasmid
    #184916
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Publication
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184916-plasmid-map-sequence-id-371408

    Enlarge

  11. pUC-dirTopo-P2A-aGFP

    Plasmid
    #61974
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61974-plasmid-map-sequence-id-101282

    Enlarge

  12. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

    Plasmid
    #171801
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Publication
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mNeonGreen-FLAG-hgp100-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    171801-plasmid-map-sequence-id-337972

    Enlarge

  14. pCRII-Topo Cdh10 in situ probe

    Plasmid
    #45638
    Depositor
    Jeffrey Macklis
    Insert
    Cdh10 in situ probe (Cdh10 Mouse)
    Use
    In situ
    Mutation
    fragment contains nt 2628-3061 of Cdh10 (GenBank …
    Available since
    June 3, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pCRISPEY-GAL-Kan-ADE2

    Plasmid
    #232105
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Publication
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232105-plasmid-map-sequence-id-462309

    Enlarge

  17. pCRISPEY-GAL-Nat-ADE2

    Plasmid
    #232103
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Publication
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232103-plasmid-map-sequence-id-461835

    Enlarge

  20. pCRISPaint-mNeon-F-gB-PuroR [M1G]

    Plasmid
    #171803
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, HSV-1 glycoprotein B CD8+ T cell epitope [AA: 498-505 (SSIEFARL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Publication
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-gB-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    171803-plasmid-map-sequence-id-341955

    Enlarge

Showing: 921 - 940 of 4673 results