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We narrowed to 63,768 results for: cloning

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Showing: 9521 - 9540 of 63768 results

  1. FB010

    Plasmid
    #119708
    Purpose
    Transcriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha1. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    119708-plasmid-map-sequence-id-236964

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  2. FB001

    Plasmid
    #119057
    Purpose
    Promoter trpC from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter trpC
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119057-plasmid-map-sequence-id-237151

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  3. FB002

    Plasmid
    #119676
    Purpose
    Terminator tub from Neurospora crassa domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Terminator tub
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119676-plasmid-map-sequence-id-237306

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  4. FB008

    Plasmid
    #119706
    Purpose
    Terminator trpC from Aspergillus nidulans domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Terminator trpC
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119706-plasmid-map-sequence-id-237146

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  5. FB012

    Plasmid
    #119709
    Purpose
    Complete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    PtrpC:hph:Ttub
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119709-plasmid-map-sequence-id-237149

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  6. FB013

    Plasmid
    #119710
    Purpose
    TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT
    Depositor
    Jose Marcos
    Publication
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
    Use
    Synthetic Biology; Domestication of dna parts for…
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119710-plasmid-map-sequence-id-237145

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  7. KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

    Plasmid
    #121813
    Purpose
    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Type
    Empty backbone
    Use
    Gateway Cloning and Synthetic Biology
    Available since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    121813-plasmid-map-sequence-id-239026

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  9. pBS_GGSG_mAID_HA_BactBsd

    Plasmid
    #235504
    Purpose
    single mAID_3xHA cloning vector with Roxed Bact-Bsd
    Depositor
    Brian Hendrich
    Insert
    mini-Auxin Inducible Degron
    Use
    Mouse Targeting
    Tags
    mAID, HA
    Mutation
    None
    Promoter
    beta actin
    Available since
    May 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    235504-plasmid-map-sequence-id-474046

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  10. mEmerald-TPX2-M1-C-18

    Plasmid
    #62764
    Purpose
    Localization: Centrosomes, C1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    TPX2M1 (TPX2 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    K313A, K314A, R315A Alanine mutagenesis
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. mEmerald-TPX2T4-C-18

    Plasmid
    #62766
    Purpose
    Localization: Centrosomes, C1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    TPX2T4 (TPX2 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Truncated TPX2 M2 AA (713-747)
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. mEmerald-TPX2-M2-C-18

    Plasmid
    #62765
    Purpose
    Localization: Centrosomes, C1 Cloning Vector, Excitation:487, Emission:509
    Depositor
    Michael Davidson
    Insert
    TPX2M2 (TPX2 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    K158A, K159A, K313A, K314A, R315A Alanine mutagen…
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pSG424 ELKC

    Plasmid
    #89333
    Purpose
    ELK1 encoding amino acid 307-428. Cloning sites BamHI and Xba I. Size 365bp
    Depositor
    Roger Davis
    Publication
    Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ELK1 (ELK1 Human)
    Tags
    Gal 4
    Expression
    Mammalian
    Mutation
    ELK1 residues 307-428
    Available since
    April 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    89333-plasmid-map-sequence-id-176434

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Showing: 9521 - 9540 of 63768 results