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p663-UBC-miniTurbo-V5-AKT1_IDG-K

Plasmid

#189890

Purpose

Gateway destination clone of AKT1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

AKT1 (AKT1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Nicastrin-CD4d3+4-bio

Plasmid

#73106

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Nicastrin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Nicastrin (NCSTN Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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GlycophorinE-CD4d3+4-bio

Plasmid

#73095

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinE with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Glycophorin E (GYPE Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK19_IDG-K

Plasmid

#158175

Purpose

Gateway destination clone of CDK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK19 (CDK19 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-CDK11B-V5-miniTurbo_IDG-K

Plasmid

#189914

Purpose

Gateway destination clone of CDK11B (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

CDK11B (CDK11B Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDK7_IDG-K

Plasmid

#158194

Purpose

Gateway destination clone of CDK7 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDK7 (CDK7 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-ERN2_IDG-K

Plasmid

#189905

Purpose

Gateway destination clone of ERN2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

ERN2 (ERN2 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-MARK3_IDG-K

Plasmid

#170583

Purpose

Gateway destination clone of MARK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-MARK3 (MARK3 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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PGRMC2-CD4d3+4-bio

Plasmid

#73112

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding PGRMC2 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

PGRMC2 (PGRMC2 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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CD200RCd4d3+4-bio

Plasmid

#32406

Purpose

A positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200 receptor) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200 Receptor extracellular domain (Cd200r1 Rat)

Use

Tags

Cd4d3+4 and biotinylation peptide

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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C8orf55-CD4d3+4-bio

Plasmid

#73126

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding C8orf55 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

C8orf55 (THEM6 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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ama1CD4d3+4-bio

Plasmid

#36145

Purpose

EXPRESs plasmid for P. falciuparum merozoite surface proteins encoding P. falciparum ama1 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ama1 (PF3D7_1133400 Plasmodium falciparum)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2012

Availability

Academic Institutions and Nonprofits only

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D44_Ld_180090

Plasmid

#135858

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

alpha glucosidase II subunit, putative

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-DSTYK _IDG-K

Plasmid

#158182

Purpose

Gateway destination clone of DSTYK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-DSTYK (DSTYK Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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p667-UBC-DYRK3-V5-miniTurbo_IDG-K

Plasmid

#158190

Purpose

Gateway destination clone of DYRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

DYRK3-V5-miniTurbo (DYRK3 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

R293H- please see depositor comments

Promoter

Ubiquitin

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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D92_Ld_360170

Plasmid

#135905

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Use

Tags

biotinylation sequence and His tag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CSNK2A2_IDG-K

Plasmid

#189870

Purpose

Gateway destination clone of CSNK2A2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

CSNK2A2 (CSNK2A2 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p663-UBC-miniTurbo-V5-DCLK3_IDG-K

Plasmid

#189891

Purpose

Gateway destination clone of DCLK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

DCLK3 (DCLK3 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p663-UBC-miniTurbo-V5-LMTK2_IDG-K

Plasmid

#170580

Purpose

Gateway destination clone of LMTK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-LMTK2 (LMTK2 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p667-UBC-HIPK3-V5-miniTurbo_IDG-K

Plasmid

#158191

Purpose

Gateway destination clone of HIPK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

HIPK3-V5-miniTurbo (HIPK3 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge