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pJOE6261.2

Plasmid

#135079

Purpose

P. putida KT2440 rescue construct

Depositor

Josef Altenbuchner

Publication

Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

Insert

upp (upp Pseudomonas putida)

Expression

Bacterial

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_PSMD1_sgRNA_1

Plasmid

#155102

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting PSMD1 (core essential gene)

Depositor

Jason Moffat

Publication

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

PSMD1_sgRNA_1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mClover3_AAVS1_sgRNA_4

Plasmid

#155101

Purpose

lentiviral plasmid expressing mClover3, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

Depositor

Jason Moffat

Publication

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_4

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO_AAVS1_sgRNA_3

Plasmid

#155086

Purpose

lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Publication

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_mCherry_AAVS1_sgRNA_4

Plasmid

#155106

Purpose

lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

Depositor

Jason Moffat

Publication

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_4

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLCKO_AAVS1_sgRNA_4

Plasmid

#155087

Purpose

lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Publication

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_4

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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LCV2_AAVS1_sgRNA_4

Plasmid

#155090

Purpose

lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

Depositor

Jason Moffat

Publication

Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

Insert

AAVS1_sgRNA_4

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 promoter

Available since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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gTBEv4

Plasmid

#220620

Purpose

vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Publication

Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:

Insert

nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)

Expression

Mammalian

Mutation

hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

Promoter

hU6, EF1a

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pExp-His-zBasic-RBD

Plasmid

#194999

Purpose

Production of SARS-CoV2 receptor binding domain in E. coli

Depositor

Marko Hyvönen

Publication

Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

Insert

RBD (S SARS-CoV-2)

Tags

His8-Zbasic

Expression

Bacterial

Promoter

T7lac

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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SpCas9-HF1

Plasmid

#138556

Purpose

Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

SpCas9-HF1

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-neo-Strep_Flag_Nterm

Plasmid

#102644

Purpose

Epitope tag n-terminus with SBP-Flag moiety

Depositor

Michael E. Wright

Publication

Hsiao et al J Biol Chem. 2016 Sep 2;291(36):18818-42

Type

Empty backbone

Tags

SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

Expression

Mammalian

Available since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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mTagRFP-pBAD

Plasmid

#54539

Purpose

Localization: Protein Expression Vector , Excitation: 555, Emission: 584

Depositor

Dmitriy Chudakov, Michael Davidson, Dorus Gadella

Publication

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Tags

6xHis and mTagRFP

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pCAG-Neo-PuroDTK

Plasmid

#84036

Purpose

PiggyBac cassette contains Neomycin, puromycin and TK genes

Depositor

Yuet Wai Kan

Publication

Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

CAG promoter

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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3XHalo_GFP_TRF1#1

Plasmid

#164644

Purpose

tag TRF1 with GFP and Halo

Depositor

Michael Lampson, Huaiying Zhang

Publication

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

TRF1 (TERF1 Human)

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFlc245

Plasmid

#101021

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Publication

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

GluR 1 (Gria1 Rat)

Expression

Mammalian

Mutation

fluorescent protein inserted into subunit

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV/3' Box_(GLuc)_TOP2

Plasmid

#68435

Purpose

Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP2 construct

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Sept. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pUBC_HA_L7Ae-VP64

Plasmid

#68421

Purpose

Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter

Depositor

John Rinn

Publication

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

L7Ae

Use

Synthetic Biology

Tags

HA and VP64

Expression

Mammalian

Promoter

human UbC

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-ELYS-emerin-EGFP-polyA

Plasmid

#59745

Purpose

Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Publication

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

ELYS-emerin-EGFP

Tags

EGFP

Expression

Mammalian

Promoter

CMV and T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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PhyB-mCherry-SIK1

Plasmid

#51577

Purpose

Constitutively express nucleolus localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Publication

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Tags

SIK1 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ146-ZmUbi-tRNA

Plasmid

#158404

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAs

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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