We narrowed to 20,084 results for: Sele

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pBiT3.1-N_ATG16L1:1-607

Plasmid

#211234

Purpose

Mammalian expression of full-length human ATG16L1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ATG16L1:M1-Y607 (ATG16L1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

T300A natural variant (VAR_021834)

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_EML4:1-981

Plasmid

#211315

Purpose

Mammalian expression of full-length human EML4. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Publication

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EML4:M1-S981 (EML4 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

I382V natural variant (VAR_031727)

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP_D2A_tdTomato

Plasmid

#184045

Purpose

Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)

Depositor

Angela Pannier

Publication

Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

Inserts

Enhanced Green Fluorescent Protein
tdTomato
Dual 2A Peptide Sequence

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_1G11F2-3 light chain

Plasmid

#198254

Purpose

recombinant expression of light chain of monoclonal anti-AMP antibody 1G11F2-3

Depositor

Aymelt Itzen

Publication

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

light chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse kappa)

Tags

IL10 signal peptide

Expression

Mammalian

Promoter

CMV

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PKCsub-YPet-NES

Plasmid

#138207

Purpose

Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES

Depositor

Jin Zhang

Publication

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

PKCsub-YPet-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

Sept. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/BCR-FGFR1

Plasmid

#167490

Purpose

Mammalian expression vector for the BCR-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

Depositor

Nicholas Cross

Publication

Demiroglu et al Blood. 2001 Dec 15;98(13):3778-83. doi:

Insert

BCR-FGFR1 (BCR Human)

Expression

Mammalian

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo-CTRLg1

Plasmid

#139450

Purpose

Lentiviral vector with non-targeting gRNA and neomycin selectable marker

Depositor

Caroline Goujon

Publication

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LentiGuide-Neo-CTRLg2

Plasmid

#139451

Purpose

Lentiviral vector with non-targeting gRNA and neomycin selectable marker

Depositor

Caroline Goujon

Publication

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

Non-targeting sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

Plasmid

#123353

Purpose

Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Publication

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-Cameleon

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Promoter

CMV

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc UNC119Aa

Plasmid

#128331

Purpose

Mammalian expression vector of Myc-tagged human UNC119A isoform a

Depositor

Yutaka Hata

Publication

Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

Insert

UNC119 (UNC119 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean-hCOL1A1

Plasmid

#140112

Purpose

Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

Depositor

Sergey Leikin

Insert

Type I procollagen α1 (COL1A1 Human)

Tags

mCerulean

Expression

Mammalian

Mutation

COL1A1 exons 2-3 replaced by fluorescent tag

Promoter

CMV

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-2xSV40_NLS-CeNLuc

Plasmid

#135952

Purpose

Expresses the CeNLuc LumiFluor targeted to the nucleus in mammalian cells.

Depositor

Antonio Amelio

Publication

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

CeNLuc LumiFluor

Tags

2xSV40-NLS

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/TO-myc-BirA*-NES

Plasmid

#112714

Purpose

For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

Depositor

Daniel Schoenberg

Publication

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

HIV Rev nuclear export signal (NES)

Expression

Mammalian

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-PU.1-ETS

Plasmid

#124652

Purpose

Chimeric ETS domain

Depositor

Gregory Poon

Publication

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (SPI1, ETS1 Human)

Expression

Mammalian

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-A2AR

Plasmid

#106066

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-A2AR; Opto-A2AR; F8; 719)

Depositor

Harald Janovjak

Publication

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-A2AR (ADORA2A Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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rat APOBEC1 E63A

Plasmid

#112861

Purpose

plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

Depositor

Silvestro Conticello

Publication

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Expression

Mammalian

Mutation

changed Glutamic acid 63 to Alanine

Promoter

CMV promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 RL3Sc

Plasmid

#109365

Purpose

Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

Depositor

Robert Lucas

Publication

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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PKAsub-TagRFPT-CAAX

Plasmid

#87712

Purpose

Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).

Depositor

Jin Zhang

Publication

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

PKAsub-TagRFPT-CAAX

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Promoter

CMV

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pUDE722

Plasmid

#103022

Purpose

expression of a Cpf1 programming crRNA targetting CAN1 (crCAN1-4S)

Depositor

Jean-Marc Daran

Publication

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crHCAN1-4.S (CAN1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Myc-CD4-mCherry-HA-FRB

Plasmid

#100740

Purpose

To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

Depositor

Stephen Royle

Publication

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CD4

Tags

FRB, HA, Myc, and mCherry

Expression

Mammalian

Mutation

Engineered to remove large part of extracellular …

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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