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pMM2-rPMCA3b

Plasmid

#47763

Purpose

mammalian expression of rat PMCA3b

Depositor

John Penniston, Emanuel Strehler

Publication

Enyedi et al J Biol Chem. 1997 Oct 31;272(44):27525-8

Insert

PMCA3b (Atp2b3 Rat)

Expression

Mammalian

Promoter

SV40

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Plexin D1 in situ probe

Plasmid

#45621

Depositor

Jeffrey Macklis

Publication

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Plexin D1 in situ probe (Plxnd1 Mouse)

Use

In situ

Mutation

fragment contains bp# 5751-6406 of NM_026376.3

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pQTEV-ILKAP

Plasmid

#34817

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

integrin-linked kinase-associated serine/threonine phosphatase 2C (ILKAP Human)

Tags

His and TEV

Expression

Bacterial

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUASp YFP Rab10 DN

Plasmid

#37709

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab10 (Rab10 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

T23N (dominant negative)

Promoter

GAL4

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pCY 3010-02

Plasmid

#36210

Depositor

Anne Robinson

Publication

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

yeast-enhanced (yE) polylinker

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-Cerulean-hphMX4

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only

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FUW-M2-PAK2 S20A

Plasmid

#31664

Depositor

Anne Brunet

Publication

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Use

Lentiviral

Tags

M2

Expression

Mammalian

Mutation

Changed Serine 20 to Alanine

Available since

April 12, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Barkor (∂CC)

Plasmid

#24404

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Barkor (ATG14 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of amino acids 91-180 of the coiled-coil…

Available since

May 20, 2010

Availability

Academic Institutions and Nonprofits only
pEF1a-mRor2deltaST1

Plasmid

#22632

Depositor

Roel Nusse

Publication

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 delta ST1 (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of ST1 domain

Available since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
pEF1a-mRor2deltaST2

Plasmid

#22633

Depositor

Roel Nusse

Publication

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 delta ST2 (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of ST2 domain

Available since

Nov. 25, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-hHIF1 alpha-IGd2

Plasmid

#22535

Purpose

To be used for the RNAi screening purposes (not for HDAC4 expression); see paper

Depositor

Connie Cepko

Publication

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

hypoxia inducible factor 1 alpha (HIF1A Human)

Expression

Mammalian

Available since

Nov. 9, 2009

Availability

Academic Institutions and Nonprofits only
mADAM8 deltaMP in pQCXIP cFLAG

Plasmid

#19149

Depositor

Zena Werb

Publication

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM8 (Adam8 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

delta 228-397

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
cmv 16 E7 C91S

Plasmid

#13689

Depositor

Karl Munger

Publication

Gonzalez et al J Virol. 2001 Aug . 75(16):7583-91.

Insert

HPV 16 E7 C91S (E7 Human)

Expression

Mammalian

Mutation

aa 91 from C to S

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pGEX2T merlin 308-579

Plasmid

#11632

Depositor

Vijaya Ramesh

Publication

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal common domain of merlin.

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pIVT_hifiDn29-dCas9-P2A-GFP

Plasmid

#247168

Purpose

In vitro transcription of human codon optimized fusion of hifiDn29-dCas9-P2A-GFP

Depositor

Patrick Hsu

Publication

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-dCas9-P2A-GFP

Use

In vitro transcription

Tags

SV40 NLS

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

mutated T7

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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Tad-CBE(TCA3)

Plasmid

#235064

Purpose

Expresses Tad-CBE(TCA3) in mammalian cells.

Depositor

Weixin Tang

Publication

Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tad-CBE(TCA3)-SpCas9 D10A

Use

CRISPR

Tags

2xUGI, bpNLS and bpNLS

Expression

Mammalian

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse)

Plasmid

#236421

Purpose

transient overexpression of KEAP1 in mammalian cells

Depositor

Michele Pagano

Publication

Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

KEAP1 (Keap1 Mouse)

Expression

Mammalian

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pLBPVXBa-M

Plasmid

#229079

Purpose

New version of a PVX vector to express heterologous sequences in plants

Depositor

Jose-Antonio Daros

Publication

Uranga et al Hortic Res. 2023 Dec 19;11(1):uhad279. d

Insert

Potato virus X

Expression

Plant

Mutation

Mutation coat protein start codon (ATG to AGG); d…

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMK463 (BSD-P2A-mAB)

Plasmid

#214389

Purpose

mAID-BromoTag for N-terminal tagging

Depositor

Masato Kanemaki

Publication

Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BSD-P2A-mAID-BromoTag

Use

CRISPR

Expression

Mammalian

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Duet1-His-TEV-mCherry-p62

Plasmid

#199780

Purpose

Used for the expression of mCherry labelled p62 (SMC Internal No. 391).

Depositor

Sascha Martens

Publication

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

6x His, TEV, mCherry

Expression

Bacterial

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCH4 (denAsCas12a-KRAB lenti)

Plasmid

#217331

Purpose

Lentiviral expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Publication

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

D908A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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