We narrowed to 12,188 results for: LEA

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pAc5.1B-EGFP-DmPAN2_377-1241_Y

Plasmid

#148030

Purpose

Insect Expression of DmPAN2_377-1241

Depositor

Elisa Izaurralde

Publication

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2_377-1241 (PAN2 Fly)

Expression

Insect

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPAN2-dsRNAres_Y

Plasmid

#148041

Purpose

Insect Expression of DmPAN2-dsRNAres

Depositor

Elisa Izaurralde

Publication

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-dsRNAres (PAN2 Fly)

Expression

Insect

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-HsNot7_9-260_R

Plasmid

#147367

Purpose

Bacterial Expression of HsNot7_9-260

Depositor

Elisa Izaurralde

Publication

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7_9-260 (CNOT7 Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsNot1iso1_1092-1317_R

Plasmid

#147368

Purpose

Bacterial Expression of HsNot1iso1_1092-1317

Depositor

Elisa Izaurralde

Publication

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1092-1317 (CNOT1 Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot7-E138AT142AE149A_R

Plasmid

#147377

Purpose

Mammalian Expression of HsNot7-E138AT142AE149A

Depositor

Elisa Izaurralde

Publication

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7-E138AT142AE149A (CNOT7 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot7-E138KT142YE149K_R

Plasmid

#147378

Purpose

Mammalian Expression of HsNot7-E138KT142YE149K

Depositor

Elisa Izaurralde

Publication

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7-E138KT142YE149K (CNOT7 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot7-M141RT142YL147R_R

Plasmid

#147380

Purpose

Mammalian Expression of HsNot7-M141RT142YL147R

Depositor

Elisa Izaurralde

Publication

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7-M141RT142YL147R (CNOT7 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot7-T142Y_R

Plasmid

#147381

Purpose

Mammalian Expression of HsNot7-T142Y

Depositor

Elisa Izaurralde

Publication

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot7-T142Y (CNOT7 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1_1595-2376_K

Plasmid

#146808

Purpose

Mammalian Expression of HsNot1iso1_1595-2376

Depositor

Elisa Izaurralde

Publication

Petit et al Nucleic Acids Res. 2012 Nov;40(21):11058

Insert

HsNot1iso1_1595-2376 (CNOT1 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST15-Eps8L2

Plasmid

#196534

Purpose

Expression of GST-Eps8L2-FL

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2 (EPS8L2 Human)

Tags

GST

Expression

Bacterial

Promoter

T7 promoter; lac UV5 promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5mycGW-Eps8L2_529-715

Plasmid

#196543

Purpose

Expression of myc-Eps8L2_529-715

Depositor

Edgar Gomes

Publication

Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

Insert

Eps8L2_529-715 (EPS8L2 Human)

Tags

myc

Expression

Mammalian

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b I-SceI (Ctermhis) K223I

Plasmid

#195615

Purpose

Expresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activity

Depositor

Frederick Gimble

Publication

Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

Insert

I-SceI

Expression

Bacterial

Mutation

C2694G, G2345C, G2351C, A3365T, A3366T

Promoter

T7

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET PI-SceI C-His K301A

Plasmid

#195611

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutation

Depositor

Frederick Gimble

Publication

He et al J Biol Chem. 1998 Feb 20;273(8):4607-15.

Insert

PI-SceI

Expression

Bacterial

Mutation

A822G, A823C, A824T

Promoter

T7

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-DmNot3_677-844_P

Plasmid

#147185

Purpose

Bacterial Expression of DmNot3_677-844

Depositor

Elisa Izaurralde

Insert

DmNot3_677-844 (Not3 Fly)

Expression

Bacterial

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmNot3_677-844_P

Plasmid

#147190

Purpose

Bacterial Expression of DmNot3_677-844

Depositor

Elisa Izaurralde

Insert

DmNot3_677-844 (Not3 Fly)

Expression

Bacterial

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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mKate2-DrCavin1a in pT3TS-Dest

Plasmid

#194294

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1a from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAI

Depositor

Rob Parton

Publication

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1a (cavin1a Zebrafish)

Use

In vitro transcription of mrna

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #2 GFP

Plasmid

#106951

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

Depositor

Albert Edge

Publication

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #3 GFP

Plasmid

#106952

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. sgRNA is less optimal. Includes GFP marker.

Depositor

Albert Edge

Publication

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2114

Plasmid

#175961

Purpose

For expression of LetA-[ΔRing6 LetB] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Gira Bhabha, Damian Ekiert

Publication

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Tags

8xHis 2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

Δ634-746

Promoter

araBAD

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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2xNLS-NLP-cMyc AspCas12a

Plasmid

#182120

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Publication

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc AspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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