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pLenti-MTS-jAspSnFR3-mRuby3

Plasmid

#203492

Purpose

Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

MTS-jAspSnFR3-mRuby3

Use

Lentiviral

Tags

His-tag, Mitochondrial localization signal, and m…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_C0000

Plasmid

#91081

Purpose

Module C, Promoter: none, Gene: none (BaeI site for GT donor cloning), Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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Marathon-RT for Split-PE (pEH59)

Plasmid

#190108

Purpose

Expresses Marathon-RT (human codon optimization)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

Marathon-RT

Tags

bpNLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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PB-GFP1-10-HygR

Plasmid

#223675

Purpose

To check the split GFP signals in mitochondria (1)

Depositor

Juan Melero-Martin

Article

Lin et al Nature. 2024 May;629(8012):660-668. doi:

Insert

GFP1-10

Expression

Mammalian

Promoter

cmv

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL848

Plasmid

#214238

Purpose

FrCas9 cytosine base editing plasmid V2.1 in rice

Depositor

Yong Zhang

Article

He et al Plant Biotechnol J. 2024 Sep;22(9):2488-

Type

Empty backbone

Expression

Plant

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher002

Plasmid

#102827

Purpose

Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyCatcher002

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pFH2.2_mCherry-MS2x12

Plasmid

#205521

Purpose

Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHK326

Plasmid

#235475

Purpose

Inducible gene VIII by Ptac promoter

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

phage M13 geneVIII

Expression

Bacterial

Available Since

May 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9-mammalian

Plasmid

#138414

Purpose

Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG, 6xHis, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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P4 cosmid

Plasmid

#196335

Purpose

P4 cosmid with CRISPR-Cas9 system

Depositor

Baojun Wang

Article

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

genes from phage P4, CRISPR-Cas9 system

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDV02

Plasmid

#212156

Purpose

Plasmid expressing secondary alcohol dehydrogenase under constitutive promoter PgyrA control.

Depositor

Kang Zhou

Article

Tan et al ACS Synth Biol. 2023 Jul 21;12(7):2041-2

Insert

secondary alcohol dehydrogenase

Expression

Bacterial

Promoter

PgyrA

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_Blinatumomab_PGK_mCherry

Plasmid

#85437

Purpose

[Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Blinatumomab

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pRVL-4

Plasmid

#104582

Purpose

Contains human kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

Depositor

Daniel Christ

Article

Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

Insert

Human CL kappa

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-NLRP10

Plasmid

#38141

Depositor

Thomas Kufer

Article

Lautz et al Cell Microbiol. 2012 Jun 4. doi: 10.1111

Insert

NLRP10 (NLRP10 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only
pFH3.2_mTagBFP2-MS2x12

Plasmid

#205558

Purpose

Express mTagBFP2-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mTagBFP2

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.10_Gag-MCP-P2A-eGFP

Plasmid

#205525

Purpose

Express HIV Gag-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pUB_smHA_KDM5B_MS2

Plasmid

#81085

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smHA_KDM5B_24xMS2

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFH2.32_LV-mCherry-MS2x8-BCS

Plasmid

#205539

Purpose

Express mCherry-MS2x8 with barcode

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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snuABE4.2

Plasmid

#246014

Purpose

snuABE4.1 with MLH1-SB fused to the C-terminus via P2A.

Depositor

Sangsu Bae

Article

Im et al bioRxiv 2025.07.19.664718

Insert

Pediculus humanus ADAR deaminase domain (E438Q, I536S, E637F mutant)

Tags

MLH1-SB

Expression

Mammalian

Mutation

E438Q, I536S, E637F, with 10 amino acids truncati…

Promoter

CMV

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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snuABE3.1

Plasmid

#246011

Purpose

snuABE3.1 composed of PhADAR(E438Q) fused to SpCas9 nickase [R221K, N394K, H840A] via an SV40 linker and further fused to La protein.

Depositor

Sangsu Bae

Article

Im et al bioRxiv 2025.07.19.664718

Insert

Pediculus humanus ADAR deaminase domain (E438Q mutant)

Expression

Mammalian

Mutation

E438Q

Promoter

CMV

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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